Document Detail


EGFR and KRAS mutational analysis and their correlation to survival in pancreatic and periampullary cancer.
MedLine Citation:
PMID:  22422135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Pancreatic and periampullary cancers have a high incidence of activating KRAS mutations. The aim of this study was to determine the incidence of KRAS and EGFR mutations in pancreatic and periampullary cancers and their relationship with survival.
METHODS: One hundred patients undergoing pancreaticoduodenectomy or pancreatic biopsy for cancer were recruited. Samples of formalin-fixed paraffin-embedded or fresh pancreatic tissue were obtained. EGFR was analyzed by DNA sequencing of exons 18 to 21. KRAS was analyzed by pyrosequencing of codons 12, 13, and 61.
RESULTS: EGFR mutations were found in 2 (2.3%) of 88 assessable cases. One in exon 18 (c.1966C>T, p.Q710X) and 1 in exon 19 (c.2066A>G, p.E734G). A synonymous single-nucleotide polymorphism in exon 20 (c.2361G>A, p.Q787) was identified in 57 (67.8%) of 84 patients studied. Twenty-eight (41.2%) of 68 cases harbored a point mutation in KRAS codon 12 (26 cases) and codon 61 (2 cases). The overall median survival was 308 days (range, 7-2623 days). The presence of KRAS point mutations did not significantly alter median survival time (22.8 vs 28.1 months, P = 0.88).
CONCLUSIONS: EGFR somatic mutations are rare in pancreatobiliary malignancies. KRAS mutations are less common than previous reports and do not correlate with survival.
Authors:
Melissa Oliveira-Cunha; Kristen D Hadfield; Ajith K Siriwardena; William Newman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pancreas     Volume:  41     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-16     Completed Date:  2012-07-31     Revised Date:  2013-04-22    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  428-34     Citation Subset:  IM    
Affiliation:
Hepatobiliary Surgery Unit, University of Manchester and Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK. melissacunha@doctors.org.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Biopsy
Codon
DNA Mutational Analysis
Digestive System Neoplasms / genetics*,  mortality*,  pathology,  surgery
England / epidemiology
Exons
Female
Genetic Predisposition to Disease
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Pancreatic Neoplasms / genetics*,  mortality*,  pathology,  surgery
Pancreaticoduodenectomy
Phenotype
Point Mutation*
Polymorphism, Single Nucleotide*
Prospective Studies
Proto-Oncogene Proteins / genetics*
Receptor, Epidermal Growth Factor / genetics*
Retrospective Studies
Time Factors
Treatment Outcome
ras Proteins / genetics*
Chemical
Reg. No./Substance:
0/Codon; 0/KRAS protein, human; 0/Proto-Oncogene Proteins; EC 2.7.10.1/EGFR protein, human; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 3.6.5.2/ras Proteins
Comments/Corrections
Comment In:
Pancreas. 2013 Apr;42(3):543-4   [PMID:  23486365 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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