Document Detail

The EGF-like homeotic protein dlk affects cell growth and interacts with growth-modulating molecules in the yeast two-hybrid system.
MedLine Citation:
PMID:  11846389     Owner:  NLM     Status:  MEDLINE    
Levels of dlk, an EGF-like homeotic protein, are critical for several differentiation processes. Because growth and differentiation are, in general, exclusive of each other, and increasing evidence indicates that Dlk1 expression changes in tumorigenic processes, we studied whether dlk could also affect cell growth. We found that, in response to glucocorticoids, Balb/c 3T3 cells with diminished levels of dlk expression develop foci-like cells that have lost contact inhibition, display altered morphology, and grow faster than control cell lines. Balb/c 3T3 cells spontaneously growing more rapidly are also dlk-negative cells. Moreover, screening by the yeast two-hybrid system, using Dlk1 constructs as baits, resulted in the isolation of GAS1 and acrogranin cDNAs. Interestingly, these proteins are cysteine-rich molecules involved in the control of cell growth. Taken together, these observations suggest that dlk may participate in a network of interactions controlling how the cells respond to growth or differentiation signals.
Victoriano Baladrón; María José Ruiz-Hidalgo; Ezio Bonvini; Elena Gubina; Vicente Notario; Jorge Laborda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  291     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-15     Completed Date:  2002-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  193-204     Citation Subset:  IM    
Laboratory of Immunobiology, Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, Rockville, Maryland 20852, USA.
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MeSH Terms
3T3 Cells
Cell Cycle Proteins
Cell Division*
Epidermal Growth Factor / genetics
Glycoproteins / chemistry,  genetics,  metabolism
Growth Substances*
Homeodomain Proteins / chemistry,  genetics,  metabolism*,  physiology*
Intercellular Signaling Peptides and Proteins*
Membrane Proteins / chemistry,  genetics,  metabolism*,  physiology*
Mice, Inbred BALB C
Protein Structure, Tertiary
RNA, Messenger / biosynthesis
Repetitive Sequences, Amino Acid
Two-Hybrid System Techniques
Yeasts / genetics
Reg. No./Substance:
0/Cell Cycle Proteins; 0/GAS1 protein, human; 0/GRN protein, human; 0/Gas1 protein, mouse; 0/Glycoproteins; 0/Grn protein, mouse; 0/Growth Substances; 0/Homeodomain Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/delta protein; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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