| EGCG inhibits mammary cancer cell migration through inhibition of nitric oxide synthase and guanylate cyclase. | |
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MedLine Citation:
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PMID: 18692479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tumor cell migration is considered as a major event in the metastatic cascade. Here we examined the effect of (-)-epigallocatechin-3-gallate (EGCG) on migration capacity and molecular mechanism using 4T1 murine mammary cancer cells as a model. Using an in vitro migration assay, we found that treatment of 4T1 cells with EGCG resulted in concentration-dependent inhibition of migration of these cells. The migration capacity of cells was reduced in presence of N(G)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of nitric oxide synthase. EGCG suppressed the elevated levels of endogenous NO/NOS in 4T1 cells and blocked the migration promoting capacity of l-arginine. Treatment with guanylate cyclase inhibitor 1-H-[1,2,4]oxadiaxolo[4,3-a]quinolalin-1-one (ODQ) reduced the migration of 4T1 cells. EGCG reduced the elevated levels of cGMP in cancer cells and blocked the migration restoring activity of 8-Br cGMP (cGMP analogue). These results indicate for the first time that EGCG inhibits mammary cancer cell migration through the inhibition of NO/NOS and guanylate cyclase. |
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Authors:
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Thejass Punathil; Trygve O Tollefsbol; Santosh K Katiyar |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-08-08 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 375 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-01 Completed Date: 2008-09-09 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 162-7 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of Alabama at Birmingham, 1670 University Boulevard, Volker Hall 557, Birmingham, AL, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticarcinogenic Agents / pharmacology* Carcinoma / enzymology*, pathology Catechin / analogs & derivatives*, pharmacology Cell Line, Tumor Cell Movement / drug effects* Cyclic GMP / metabolism Enzyme Inhibitors / pharmacology Guanylate Cyclase / antagonists & inhibitors* Mammary Neoplasms, Animal / enzymology*, pathology Mice NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / antagonists & inhibitors* Nitrogen Oxides / antagonists & inhibitors, metabolism Oxadiazoles / pharmacology Quinoxalines / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P30 AR050948-010001/AR/NIAMS NIH HHS; R01 CA129415/CA/NCI NIH HHS; R01 CA129415-01A1/CA/NCI NIH HHS; R01 CA129415-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; 0/Anticarcinogenic Agents; 0/Enzyme Inhibitors; 0/Nitrogen Oxides; 0/Oxadiazoles; 0/Quinoxalines; 154-23-4/Catechin; 50903-99-6/NG-Nitroarginine Methyl Ester; 7665-99-8/Cyclic GMP; 989-51-5/epigallocatechin gallate; EC 1.14.13.39/Nitric Oxide Synthase; EC 4.6.1.2/Guanylate Cyclase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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