| EDC-mediated DNA attachment to nanocrystalline CVD diamond films. | |
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MedLine Citation:
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PMID: 16442276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemical vapour deposited (CVD) diamond is a very promising material for biosensor fabrication owing both to its chemical inertness and the ability to make it electrical semiconducting that allows for connection with integrated circuits. For biosensor construction, a biochemical method to immobilize nucleic acids to a diamond surface has been developed. Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition (MPECVD). After hydrogenation of the surface, 10-undecenoic acid, an omega-unsaturated fatty acid, is tethered by 254 nm photochemical attachment. This is followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide (EDC)-mediated attachment of amino (NH(2))-modified dsDNA. The functionality of the covalently bound dsDNA molecules is confirmed by fluorescence measurements, PCR and gel electrophoresis during 35 denaturation and rehybridisation steps. The linking method after the fatty acid attachment can easily be applied to other biomolecules like antibodies and enzymes. |
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Authors:
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P Christiaens; V Vermeeren; S Wenmackers; M Daenen; K Haenen; M Nesládek; M vandeVen; M Ameloot; L Michiels; P Wagner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-24 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 22 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-25 Completed Date: 2007-10-19 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 170-7 Citation Subset: IM |
Affiliation:
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Hasselt University, Institute for Materials Research and Transnationale Universiteit Limburg, School for Life Sciences, Wetenschapspark 1, B-3590 Diepenbeek, Belgium. Pieter.Christiaens@uhasselt.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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instrumentation* DNA* / chemical synthesis Diamond* Ethyldimethylaminopropyl Carbodiimide* Fatty Acids, Unsaturated Nanoparticles* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 1892-57-5/Ethyldimethylaminopropyl Carbodiimide; 7782-40-3/Diamond; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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