Document Detail


ED(27) trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype.
MedLine Citation:
PMID:  11869090     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ED(27) trophoblast-like cells were prepared from human chorionic villus samples obtained at 9 weeks gestation and have been grown continuously in vitro without phenotypic drift for nearly a decade. These cells express many trophoblast markers, including cytokeratin, placental alkaline phosphatase (PLAP), secretion of 17beta-estradiol, and a microvillous apical surface. The ED(27) cell line is a useful model system for studies of placental cell biology and has been distributed to laboratories world-wide. However, experiments to investigate their relationship to primary villous cytotrophoblast have shown that these cells do not secrete detectable amounts of human chorionic gonadotropin in culture and, when digested with trypsin, disperse into individual cells. Furthermore, immunocytochemical studies demonstrated that, unlike villous cytotrophoblasts, ED(27) cells were immunoreactive with monoclonal antibodies recognizing some HLA Class I antigens. This was not HLA-G, however, as would be expected if these cells originated from extravillous cytotrophoblasts, but rather classical HLA-A, B which is thought not to be expressed by any trophoblast subpopulations. These inconsistencies prompted us to question the authenticity of the continuous cell line as it now exists. Genetic haplotype analysis using the polymerase chain reaction (PCR) revealed that ED(27) was genetically identically to the HeLa cell line. Inasmuch as HeLa cells have never been grown in the laboratory (DAK), the only possible origin of HeLa cell contamination of ED(27) cells was the WISH cell line, and further PCR analysis revealed that this cell line was also genetically identical to HeLa. Like ED(27) cells, HeLa cells and WISH cells synthesized small amounts of estrogen and were found to express PLAP and antigens recognized by the monoclonal antibodies ED822, directed against the syncytiotrophoblast, and J1B5 directed against villous cytotrophoblast. These results point out the need for adherence to rigorous and consistent quality control measures to assure the authenticity of cell lines used as in vitro model systems.
Authors:
D A Kniss; Y Xie; Y Li; S Kumar; E A Linton; P Cohen; P Fan-Havard; C W G Redman; I L Sargent
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Placenta     Volume:  23     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-28     Completed Date:  2002-04-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  32-43     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Harcourt Publishers Ltd.
Affiliation:
Department of Obstetrics and Gynecology (Laboratory of Perinatal Research and Division of Maternal-Fetal Medicine), The Ohio State University, Colleges of Medicine and Public Health, Engineering and Pharmacy, Columbus, Ohio 43210, USA. kniss.1@osu.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / analysis
Cell Line, Transformed / cytology,  immunology
Chorionic Villi / immunology
Culture Techniques / standards
DNA / analysis
Equipment Contamination
Female
Flow Cytometry
Haplotypes
Hela Cells / cytology*,  immunology
Histocompatibility Antigens Class I / analysis
Humans
Immunohistochemistry
Phenotype
Polymerase Chain Reaction
Pregnancy
Pregnancy Trimester, First
Trophoblasts / cytology*,  immunology
Grant Support
ID/Acronym/Agency:
R01 HD35881/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Histocompatibility Antigens Class I; 9007-49-2/DNA
Comments/Corrections
Comment In:
Placenta. 2002 Jan;23(1):2   [PMID:  11869087 ]

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