| EBP50 gene transfection promotes 5-fluorouracil-induced apoptosis in gastric cancer cells through Bax- and Bcl-2-triggered mitochondrial pathways. | |
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MedLine Citation:
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PMID: 22366766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5-Fluorouracil (5-FU) plays an important role in the chemotherapy of advanced gastric cancer. However, genetic factors that affect therapeutic efficacy of 5-FU warrant further investigation. In the present study, using stable transfection of the ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) gene, we explored the genetic influences on 5-FU-induced apoptosis of human gastric cancer cells. Stable overexpression of the EBP50 gene was determined by reverse transcription polymerase chain reaction (RT-PCR) assay and western blot analysis. After treatment with 5-FU, cell growth activities in vitro were investigated by MTT assay. Cell apoptosis was evaluated by Hoechst 33258 staining and flow cytometry of Annexin V-FITC/PI staining. Compared with the BGC823 or BGC823/neo cells, EBP50 mRNA and protein levels in the BGC823/EBP50 cells (EBP50-transfected BGC823 cells) were markedly higher. Chemosensitivity and apoptosis rates of the BGC823/EBP50 cells were higher compared to the BGC823 and BGC823/neo cells following treatment with 5-FU. Stable overexpression of extrinsic EBP50 distinctly increases the 5-FU-induced apoptosis of gastric cancer cells, and is a novel strategy by which to improve the chemosensitivity of gastric cancer to 5-FU. |
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Authors:
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Xiao-Guang Lv; Meng-Yao Ji; Wei-Guo Dong; Xiao-Fei Lei; Meng Liu; Xu-Feng Guo; Jing Wang; Chuo Fang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-02-22 |
Journal Detail:
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Title: Molecular medicine reports Volume: 5 ISSN: 1791-3004 ISO Abbreviation: Mol Med Rep Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-03-12 Completed Date: 2012-07-03 Revised Date: 2013-02-22 |
Medline Journal Info:
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Nlm Unique ID: 101475259 Medline TA: Mol Med Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 1220-6 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antimetabolites, Antineoplastic
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pharmacology* Apoptosis / drug effects*, genetics* Cell Line, Tumor Drug Resistance, Neoplasm / drug effects, genetics Fluorouracil / pharmacology* Humans Mitochondria / genetics, metabolism*, pathology Phosphoproteins / biosynthesis*, genetics Sodium-Hydrogen Antiporter / biosynthesis*, genetics Stomach Neoplasms / genetics, metabolism, therapy* Transfection bcl-2-Associated X Protein / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites, Antineoplastic; 0/Phosphoproteins; 0/Sodium-Hydrogen Antiporter; 0/bcl-2-Associated X Protein; 0/sodium-hydrogen exchanger regulatory factor; 51-21-8/Fluorouracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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