Document Detail

EBP50 gene transfection promotes 5-fluorouracil-induced apoptosis in gastric cancer cells through Bax- and Bcl-2-triggered mitochondrial pathways.
MedLine Citation:
PMID:  22366766     Owner:  NLM     Status:  MEDLINE    
5-Fluorouracil (5-FU) plays an important role in the chemotherapy of advanced gastric cancer. However, genetic factors that affect therapeutic efficacy of 5-FU warrant further investigation. In the present study, using stable transfection of the ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) gene, we explored the genetic influences on 5-FU-induced apoptosis of human gastric cancer cells. Stable overexpression of the EBP50 gene was determined by reverse transcription polymerase chain reaction (RT-PCR) assay and western blot analysis. After treatment with 5-FU, cell growth activities in vitro were investigated by MTT assay. Cell apoptosis was evaluated by Hoechst 33258 staining and flow cytometry of Annexin V-FITC/PI staining. Compared with the BGC823 or BGC823/neo cells, EBP50 mRNA and protein levels in the BGC823/EBP50 cells (EBP50-transfected BGC823 cells) were markedly higher. Chemosensitivity and apoptosis rates of the BGC823/EBP50 cells were higher compared to the BGC823 and BGC823/neo cells following treatment with 5-FU. Stable overexpression of extrinsic EBP50 distinctly increases the 5-FU-induced apoptosis of gastric cancer cells, and is a novel strategy by which to improve the chemosensitivity of gastric cancer to 5-FU.
Xiao-Guang Lv; Meng-Yao Ji; Wei-Guo Dong; Xiao-Fei Lei; Meng Liu; Xu-Feng Guo; Jing Wang; Chuo Fang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-22
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-07-03     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1220-6     Citation Subset:  IM    
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
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MeSH Terms
Antimetabolites, Antineoplastic / pharmacology*
Apoptosis / drug effects*,  genetics*
Cell Line, Tumor
Drug Resistance, Neoplasm / drug effects,  genetics
Fluorouracil / pharmacology*
Mitochondria / genetics,  metabolism*,  pathology
Phosphoproteins / biosynthesis*,  genetics
Sodium-Hydrogen Antiporter / biosynthesis*,  genetics
Stomach Neoplasms / genetics,  metabolism,  therapy*
bcl-2-Associated X Protein / biosynthesis*,  genetics
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Phosphoproteins; 0/Sodium-Hydrogen Antiporter; 0/bcl-2-Associated X Protein; 0/sodium-hydrogen exchanger regulatory factor; 51-21-8/Fluorouracil

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