Document Detail

E74-like factor 2 regulates valosin-containing protein expression.
MedLine Citation:
PMID:  17368566     Owner:  NLM     Status:  MEDLINE    
Enhanced expression of valosin-containing protein (VCP) correlates with invasion and metastasis of cancers. To clarify the transcription mechanism of VCP, human and mouse genomic sequence was compared, revealing a 260 bp DNA sequence in the 5'-flanking region of VCP gene to be highly conserved between the two, in which binding motif of E74-like factor 2/new Ets-related factor (ELF2/NERF) was identified. Chromatin immunoprecipitation assay showed binding of ELF2/NERF to the 5'-flanking region of VCP gene. Knock-down of ELF2/NERF by siRNA decreased expression level of VCP. Viability of cells under tumor necrosis factor-alpha treatment significantly reduced in ELF2/NERF-knock-down breast cancer cell line. Immunohistochemical analysis on clinical breast cancer specimens showed a correlation of nuclear ELF2/NERF expression with VCP expression and proliferative activity of cells shown by Ki-67 immunohistochemistry. These findings indicate that ELF2/NERF promotes VCP transcription and that ELF2/NERF-VCP pathway might be important for cell survival and proliferation under cytokine stress.
Binglin Zhang; Yasuhiko Tomita; Ying Qiu; Juxiang He; Eiichi Morii; Shinzaburo Noguchi; Katsuyuki Aozasa
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Publication Detail:
Type:  Journal Article     Date:  2007-03-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  356     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-05     Completed Date:  2007-06-06     Revised Date:  2009-09-03    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  536-41     Citation Subset:  IM    
Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
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MeSH Terms
5' Flanking Region / physiology
Adenosine Triphosphatases / biosynthesis*
Aged, 80 and over
Apoptosis / physiology
Base Sequence
Breast Neoplasms / metabolism
Carcinoma / metabolism
Cell Cycle Proteins / biosynthesis*
Cell Line, Tumor
Cell Proliferation / drug effects
Chromatin Immunoprecipitation
Gene Expression Regulation
Ki-67 Antigen / biosynthesis
Middle Aged
Molecular Sequence Data
RNA Interference
Sequence Alignment
Transcription Factors / physiology*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Ki-67 Antigen; 0/Transcription Factors; 181233-60-3/ELF2 protein, human; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/CDC48 protein

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