Document Detail


E2F8 contributes to human hepatocellular carcinoma via regulating cell proliferation.
MedLine Citation:
PMID:  20068156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The E2F family member of transcription factors includes the atypical member E2F8, which has been little studied in cancer. We report that E2F8 is strongly upregulated in human hepatocellular carcinoma (HCC), where it was evidenced to contribute to oncogenesis and progression. Ectopic overexpression of E2F8 promoted cell proliferation, colony formation, and tumorigenicity, whereas E2F8 knockdown inhibited these phenotypes, as documented in Huh-7, Focus, Hep3B, and YY-8103 HCC cell lines. Mechanistic analyses indicated that E2F8 could bind to regulatory elements of cyclin D1, regulating its transcription and promoting accumulation of S-phase cells. Together, our findings suggest that E2F8 contributes to the oncogenic potential of HCC and may constitute a potential therapeutic target in this disease.
Authors:
Qing Deng; Qun Wang; Wei-Ying Zong; Da-Li Zheng; Yi-Xin Wen; Ke-Sheng Wang; Xiao-Mei Teng; Xin Zhang; Jian Huang; Ze-Guang Han
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-12
Journal Detail:
Title:  Cancer research     Volume:  70     ISSN:  1538-7445     ISO Abbreviation:  Cancer Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-15     Completed Date:  2010-03-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  782-91     Citation Subset:  IM    
Affiliation:
National Human Genome Center, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201203, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular / genetics,  metabolism,  pathology*
Cell Growth Processes / physiology
Cell Line, Tumor
Cyclin D1 / biosynthesis,  genetics
G1 Phase / physiology
Humans
Liver Neoplasms / genetics,  metabolism,  pathology*
Male
Mice
Mice, Inbred BALB C
Mice, Inbred NOD
Mice, SCID
RNA, Small Interfering / administration & dosage,  genetics
Repressor Proteins / biosynthesis*,  genetics
S Phase / physiology
Transfection
Up-Regulation
Chemical
Reg. No./Substance:
0/E2F8 protein, human; 0/RNA, Small Interfering; 0/Repressor Proteins; 136601-57-5/Cyclin D1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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