Document Detail


E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control.
MedLine Citation:
PMID:  11030352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
E2F transcription factors are major regulators of cell proliferation. The diversity of the E2F family suggests that individual members perform distinct functions in cell cycle control. E2F4 and E2F5 constitute a defined subset of the family. Until now, there has been little understanding of their individual biochemical and biological functions. Here, we report that simultaneous inactivation of E2F4 and E2F5 in mice results in neonatal lethality, suggesting that they perform overlapping functions during mouse development. Embryonic fibroblasts isolated from these mice proliferated normally and reentered from Go with normal kinetics compared to wild-type cells. However, they failed to arrest in G1 in response to p16INK4a. Thus, E2F4 and E2F5 are dispensable for cell cycle progression but necessary for pocket protein-mediated G1 arrest of cycling cells.
Authors:
S Gaubatz; G J Lindeman; S Ishida; L Jakoi; J R Nevins; D M Livingston; R E Rempel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular cell     Volume:  6     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  729-35     Citation Subset:  IM    
Affiliation:
The Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Carrier Proteins / genetics
Cell Survival / genetics
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / genetics
DNA-Binding Proteins / genetics*,  metabolism
E2F4 Transcription Factor
E2F5 Transcription Factor
Fibroblasts / cytology
G1 Phase / physiology*
Gene Expression Regulation, Developmental
Genes, ras / physiology
Genotype
Heterozygote
Homozygote
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Nuclear Proteins / genetics*,  metabolism
Phenotype
Phosphoproteins / genetics*,  metabolism
Phosphorylation
Proteins*
Retinoblastoma Protein / genetics,  metabolism
Retinoblastoma-Like Protein p107
Retinoblastoma-Like Protein p130
Transcription Factors / genetics*,  metabolism
Transcription, Genetic / physiology
Transfection
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA-Binding Proteins; 0/E2F4 Transcription Factor; 0/E2F5 Transcription Factor; 0/E2f5 protein, mouse; 0/Nuclear Proteins; 0/Phosphoproteins; 0/Proteins; 0/Rbl1 protein, mouse; 0/Rbl2 protein, mouse; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p107; 0/Retinoblastoma-Like Protein p130; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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