| E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control. | |
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MedLine Citation:
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PMID: 11030352 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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E2F transcription factors are major regulators of cell proliferation. The diversity of the E2F family suggests that individual members perform distinct functions in cell cycle control. E2F4 and E2F5 constitute a defined subset of the family. Until now, there has been little understanding of their individual biochemical and biological functions. Here, we report that simultaneous inactivation of E2F4 and E2F5 in mice results in neonatal lethality, suggesting that they perform overlapping functions during mouse development. Embryonic fibroblasts isolated from these mice proliferated normally and reentered from Go with normal kinetics compared to wild-type cells. However, they failed to arrest in G1 in response to p16INK4a. Thus, E2F4 and E2F5 are dispensable for cell cycle progression but necessary for pocket protein-mediated G1 arrest of cycling cells. |
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Authors:
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S Gaubatz; G J Lindeman; S Ishida; L Jakoi; J R Nevins; D M Livingston; R E Rempel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular cell Volume: 6 ISSN: 1097-2765 ISO Abbreviation: Mol. Cell Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-11-03 Completed Date: 2000-11-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 729-35 Citation Subset: IM |
Affiliation:
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The Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Carrier Proteins / genetics Cell Survival / genetics Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p21 Cyclins / genetics DNA-Binding Proteins / genetics*, metabolism E2F4 Transcription Factor E2F5 Transcription Factor Fibroblasts / cytology G1 Phase / physiology* Gene Expression Regulation, Developmental Genes, ras / physiology Genotype Heterozygote Homozygote Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Nuclear Proteins / genetics*, metabolism Phenotype Phosphoproteins / genetics*, metabolism Phosphorylation Proteins* Retinoblastoma Protein / genetics, metabolism Retinoblastoma-Like Protein p107 Retinoblastoma-Like Protein p130 Transcription Factors / genetics*, metabolism Transcription, Genetic / physiology Transfection |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA-Binding Proteins; 0/E2F4 Transcription Factor; 0/E2F5 Transcription Factor; 0/E2f5 protein, mouse; 0/Nuclear Proteins; 0/Phosphoproteins; 0/Proteins; 0/Rbl1 protein, mouse; 0/Rbl2 protein, mouse; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p107; 0/Retinoblastoma-Like Protein p130; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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