Document Detail


E2F transcription factors and pRb pocket proteins in cell cycle progression.
MedLine Citation:
PMID:  15576936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The E2F-family of transcripion factors exerts fascinating and contrasting functions in transcriptional repression and activation of genes regulating proliferation, apoptosis, and differentiation. E2F is principally regulated by its temporal association with retinoblastoma pocket protein (pRb) family members. In turn, pRb is regulated through phosphorylation by cyclin-dependent kinase (cdk). The activity of cdk is negatively regulated by cdk-inhibitors, exemplified by p16INK4a, p21CIP1, and p27KIP1. Therefore, positive and negative signaling events converge on E2F activity resulting in distinct growth-controling and apoptotic activities. Here we describe the immunocytochemical detection of E2F, genomic DNA, BrdU-incorporation, and mitosis in cardiomyoctes. A detailed protocol is given to illustrate this technique in primary heart muscle cells.
Authors:
Ludger Hauck; Rüdiger von Harsdorf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  296     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-03-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-45     Citation Subset:  IM    
Affiliation:
Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Bromodeoxyuridine
Cell Cycle / physiology*
Cell Cycle Proteins / physiology*
Cell Separation / methods
Cells, Cultured
Cells, Immobilized
DNA-Binding Proteins / physiology*
E2F Transcription Factors
Fluorescent Antibody Technique, Indirect / methods
Immunohistochemistry / methods
Myocytes, Cardiac / cytology,  metabolism
Rats
Retinoblastoma Protein / physiology*
Transcription Factors / physiology*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/Retinoblastoma Protein; 0/Transcription Factors; 59-14-3/Bromodeoxyuridine

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