Document Detail


E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells.
MedLine Citation:
PMID:  18778741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell density is an inherent constraint in commercial mammalian cell cultures. Here, we describe a cell engineering strategy utilizing the overexpression of the E2F-1 cell cycle transcription factor in CHO DG44 cells that produce a monoclonal antibody in serum-free, suspension culture. Stable pools and cell lines expressing E2F-1 were isolated that attained viable cell densities 20% higher than control cell lines and continued proliferation for an additional day in batch culture. There were no significant changes in antibody production, apoptosis, and cell cycle compared to control cells, nor were the growth effects evident in fed-batch conditions. Overall, E2F-1 overexpression postponed entry into stationary phase in mammalian cells, but perhaps novel E2F-1 variants or combination cell cycle engineering strategies will be necessary to realize significant growth benefits in commercial applications.
Authors:
Brian S Majors; Nilou Arden; George A Oyler; Gisela G Chiang; Nels E Pederson; Michael J Betenbaugh
Publication Detail:
Type:  Journal Article     Date:  2008-08-20
Journal Detail:
Title:  Journal of biotechnology     Volume:  138     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-10     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  103-6     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, 221 Maryland Hall, Baltimore, MD 21218-2694, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
CHO Cells
Cell Count
Cell Culture Techniques
Cell Survival
Cricetinae
Cricetulus
E2F1 Transcription Factor / metabolism*
Gene Expression*
Humans
Chemical
Reg. No./Substance:
0/E2F1 Transcription Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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