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E2A basic-helix-loop-helix transcription factors are negatively regulated by serum growth factors and by the Id3 protein.
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MedLine Citation:
PMID:  8759016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Id3, a member of the Id multigene family of dominant negative helix-loop-helix transcription factors, is induced sharply in murine fibroblasts by serum growth factors. To identify relevant targets of Id3 activity, the yeast two-hybrid system was used to identify proteins that dimerize with Id3. Four murine cDNAs were identified in the screen, all of which encode helix-loop-helix proteins: E12, E47, ALF1 and Id4. Co-immunoprecipitation assays confirm that Id3 interacts with E12, E47 and two alternative splice products of ALF1 in vitro. Id3 disrupts DNA binding by these proteins in vitro and blocks transcriptional activation by these factors in cultured murine cells. Additionally, Id3 shows evidence of interacting with the related proteins E2-2 and MyoD, but not c-Myc. These results suggest that Id3 can function as a general negative regulator of the basic-helix-loop-helix family of transcription factors exemplified by the 'E' proteins and MyoD. Although it was previously suspected that E2A is constitutively expressed, our data indicate that E2A is induced in quiescent fibroblasts, by growth factor withdrawal but not by contact inhibition of cell proliferation. These observations extend the role of Id3 in the functional antagonism of E2A-class transcription factors, and suggest that E2A proteins may mediate growth inhibition.
Authors:
D A Loveys; M B Streiff; G J Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  24     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-09-17     Completed Date:  1996-09-17     Revised Date:  2009-11-23    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2813-20     Citation Subset:  IM    
Affiliation:
Division of Pediatric Hematology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Base Sequence
Basic Helix-Loop-Helix Transcription Factors
Cell Cycle
Cell Division
DNA Primers
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation
Growth Substances / metabolism
Helix-Loop-Helix Motifs*
Humans
Inhibitor of Differentiation Proteins
Mice
Molecular Sequence Data
MyoD Protein / genetics,  metabolism
Neoplasm Proteins*
Protein Binding
TCF Transcription Factors
Transcription Factors / chemistry,  genetics,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
CA01622/CA/NCI NIH HHS; HD27799/HD/NICHD NIH HHS; HL07525/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Growth Substances; 0/Inhibitor of Differentiation Proteins; 0/MyoD Protein; 0/Neoplasm Proteins; 0/TCF Transcription Factors; 0/Tcf12 protein, mouse; 0/Transcription Factors; 0/transcription factor 7-like 1 protein; 142661-93-6/TCF12 protein, human; 147785-34-0/ID3 protein, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 15 Month: 7 Year: 1996
Volume: 24 Issue: 14
First Page: 2813 Last Page: 2820
ID: 145994
PubMed Id: 8759016
Publisher Item Identifier: 6g0058

E2A basic-helix-loop-helix transcription factors are negatively regulated by serum growth factors and by the Id3 protein.
D A Loveys
M B Streiff
G J Kato
Division of Pediatric Hematology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.


Article Categories:
  • Research Article


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