Document Detail

E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA.
MedLine Citation:
PMID:  222478     Owner:  NLM     Status:  MEDLINE    
The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis. The maximal density of supercoiling is obtained at a HU-DNA mass ratio of 1. Reconstituted DNA-HU complexes prefixed with glutaraldehyde appear as condensed circular structures having an average of 14 "beads" per circular SV40 DNA molecule, with a "bead" diameter of 180 +/- 23 A. The circular SV40 DNA is condensed by a ratio of 2.0-2.5 relative to naked DNA. This is similar to the ratio (2.4) measured for chromatin formed by reassociation of relaxed SV40 DNA with the four core histones.
J Rouvière-Yaniv; M Yaniv; J E Germond
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell     Volume:  17     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-09-25     Completed Date:  1979-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  265-74     Citation Subset:  IM    
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MeSH Terms
Chromatin / metabolism,  ultrastructure*
DNA Helicases / metabolism*
DNA, Superhelical / metabolism*
DNA, Viral / metabolism
Endonucleases / metabolism
Escherichia coli
Histones / metabolism
Isoelectric Point
Molecular Weight
Simian virus 40
Structure-Activity Relationship
Reg. No./Substance:
0/Chromatin; 0/DNA, Superhelical; 0/DNA, Viral; 0/Histones; EC 3.1.-/Endonucleases; EC 3.6.1.-/DNA Helicases

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