Document Detail


E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA.
MedLine Citation:
PMID:  222478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis. The maximal density of supercoiling is obtained at a HU-DNA mass ratio of 1. Reconstituted DNA-HU complexes prefixed with glutaraldehyde appear as condensed circular structures having an average of 14 "beads" per circular SV40 DNA molecule, with a "bead" diameter of 180 +/- 23 A. The circular SV40 DNA is condensed by a ratio of 2.0-2.5 relative to naked DNA. This is similar to the ratio (2.4) measured for chromatin formed by reassociation of relaxed SV40 DNA with the four core histones.
Authors:
J Rouvière-Yaniv; M Yaniv; J E Germond
Related Documents :
10805748 - Interference of the simian virus 40 origin of replication by the cytomegalovirus immedi...
6258398 - Complete in vitro dna replication of sv40 chromatin in digitonin-treated permeable cells.
2998028 - Electron microscopic evidence for the cruciform structure in intracellular sv40 dna.
8382398 - Lytic functions of mutant polyomavirus large t-antigen with deletion of retinoblastoma ...
16793378 - Characterization of escherichia coli translesion synthesis polymerases and their access...
18497088 - Dynamics of oxidative damage at early stages of estrogen-dependant carcinogenesis.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell     Volume:  17     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-09-25     Completed Date:  1979-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  265-74     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatin / metabolism,  ultrastructure*
DNA Helicases / metabolism*
DNA, Superhelical / metabolism*
DNA, Viral / metabolism
Endonucleases / metabolism
Escherichia coli
Histones / metabolism
Isoelectric Point
Molecular Weight
Simian virus 40
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA, Superhelical; 0/DNA, Viral; 0/Histones; EC 3.1.-/Endonucleases; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The introduction of poliovirus RNA into cells via lipid vesicles (liposomes).
Next Document:  A murine sarcoma virus-associated protein kinase: interaction with actin and microtubular protein.