Document Detail


E-cadherin plays an essential role in collective directional migration of large epithelial sheets.
MedLine Citation:
PMID:  22410739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In wound healing and development, large epithelial sheets migrate collectively, in defined directions, and maintain tight cell-cell adhesion. This type of movement ensures an essential function of epithelia, a barrier, which is lost when cells lose connection and move in isolation. Unless wounded, epithelial sheets in cultures normally do not have overall directional migration. Cell migration is mostly studied when cells are in isolation and in the absence of mature cell-cell adhesion; the mechanisms of the migration of epithelial sheets are less well understood. We used small electric fields (EFs) as a directional cue to instigate and guide migration of epithelial sheets. Significantly, cells in monolayer migrated far more efficiently and directionally than cells in isolation or smaller cell clusters. We demonstrated for the first time the group size-dependent directional migratory response in several types of epithelial cells. Gap junctions made a minimal contribution to the directional collective migration. Breaking down calcium-dependent cell-cell adhesion significantly reduced directional sheet migration. Furthermore, E-cadherin blocking antibodies abolished migration of cell sheets. Traction force analysis revealed an important role of forces that cells in the leading rows exert on the substratum. With EF, the traction forces of the leading edge cells coordinated in directional re-orientation. Our study thus identifies a novel mechanism--E-cadherin dependence and coordinated traction forces of leading cells in collective directional migration of large epithelial sheets.
Authors:
Li Li; Robert Hartley; Bjoern Reiss; Yaohui Sun; Jin Pu; Dan Wu; Francis Lin; Trung Hoang; Soichiro Yamada; Jianxin Jiang; Min Zhao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-03-13
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  69     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-19     Completed Date:  2012-09-27     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  2779-89     Citation Subset:  IM    
Affiliation:
Department of Dermatology, School of Medicine, Institute for Regenerative Cures, University of California at Davis, Suite 1630, 2921 Stockton Blvd., Room 1617, Sacramento, CA 95817, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadherins / metabolism*
Cattle
Cell Adhesion
Cell Communication
Cell Movement / physiology*
Cells, Cultured
Dogs
Electric Conductivity
Epithelium, Corneal / cytology,  metabolism*
Gap Junctions / physiology*
Kidney / cytology,  metabolism*
Rats
Trachea / cytology,  metabolism*
Grant Support
ID/Acronym/Agency:
1R01EY019101/EY/NEI NIH HHS; R01 EY019101/EY/NEI NIH HHS; R01 GM094798/GM/NIGMS NIH HHS; R01GM094798/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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