Document Detail

An E-cadherin-mediated hitchhiking mechanism for C. elegans germ cell internalization during gastrulation.
MedLine Citation:
PMID:  22675206     Owner:  NLM     Status:  MEDLINE    
Gastrulation movements place endodermal precursors, mesodermal precursors and primordial germ cells (PGCs) into the interior of the embryo. Somatic cell gastrulation movements are regulated by transcription factors that also control cell fate, coupling cell identity and position. By contrast, PGCs in many species are transcriptionally quiescent, suggesting that they might use alternative gastrulation strategies. Here, we show that C. elegans PGCs internalize by attaching to internal endodermal cells, which undergo morphogenetic movements that pull the PGCs into the embryo. We show that PGCs enrich HMR-1/E-cadherin at their surfaces to stick to endoderm. HMR-1 expression in PGCs is necessary and sufficient to ensure internalization, suggesting that HMR-1 can promote PGC-endoderm adhesion through a mechanism other than homotypic trans interactions between the two cell groups. Finally, we demonstrate that the hmr-1 3' untranslated region promotes increased HMR-1 translation in PGCs. Our findings reveal that quiescent PGCs employ a post-transcriptionally regulated hitchhiking mechanism to internalize during gastrulation, and demonstrate a morphogenetic role for the conserved association of PGCs with the endoderm.
Daisuke Chihara; Jeremy Nance
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-06
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-27     Completed Date:  2012-09-04     Revised Date:  2013-07-17    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2547-56     Citation Subset:  IM    
Helen L. and Martin S. Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, NYU School of Medicine, New York, NY 10016, USA.
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MeSH Terms
3' Untranslated Regions / genetics
Cadherins / genetics,  metabolism*
Caenorhabditis elegans / cytology*,  metabolism*
Cell Adhesion / physiology
Endoderm / metabolism
Gastrulation / genetics,  physiology*
Germ Cells / cytology*,  metabolism*
In Situ Hybridization
Grant Support
Reg. No./Substance:
0/3' Untranslated Regions; 0/Cadherins

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