Document Detail


An E-cadherin-mediated hitchhiking mechanism for C. elegans germ cell internalization during gastrulation.
MedLine Citation:
PMID:  22675206     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gastrulation movements place endodermal precursors, mesodermal precursors and primordial germ cells (PGCs) into the interior of the embryo. Somatic cell gastrulation movements are regulated by transcription factors that also control cell fate, coupling cell identity and position. By contrast, PGCs in many species are transcriptionally quiescent, suggesting that they might use alternative gastrulation strategies. Here, we show that C. elegans PGCs internalize by attaching to internal endodermal cells, which undergo morphogenetic movements that pull the PGCs into the embryo. We show that PGCs enrich HMR-1/E-cadherin at their surfaces to stick to endoderm. HMR-1 expression in PGCs is necessary and sufficient to ensure internalization, suggesting that HMR-1 can promote PGC-endoderm adhesion through a mechanism other than homotypic trans interactions between the two cell groups. Finally, we demonstrate that the hmr-1 3' untranslated region promotes increased HMR-1 translation in PGCs. Our findings reveal that quiescent PGCs employ a post-transcriptionally regulated hitchhiking mechanism to internalize during gastrulation, and demonstrate a morphogenetic role for the conserved association of PGCs with the endoderm.
Authors:
Daisuke Chihara; Jeremy Nance
Related Documents :
18234566 - Vitamin c supply to bronchial epithelial cells linked to glutathione availability in el...
9922216 - Gene transfer of mitochondrially targeted glutathione reductase protects h441 cells fro...
18023956 - Acrolein oxidizes the cytosolic and mitochondrial thioredoxins in human endothelial cells.
7859346 - Expression of gamma-glutamyl transpeptidase provides tumor cells with a selective growt...
2993316 - Intracellular ph in dictyostelium discoideum: a 31p nuclear magnetic resonance study.
9004416 - The extracellular matrix in neural crest-cell migration.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-06
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-27     Completed Date:  2012-09-04     Revised Date:  2013-07-17    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2547-56     Citation Subset:  IM    
Affiliation:
Helen L. and Martin S. Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, NYU School of Medicine, New York, NY 10016, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions / genetics
Animals
Cadherins / genetics,  metabolism*
Caenorhabditis elegans / cytology*,  metabolism*
Cell Adhesion / physiology
Endoderm / metabolism
Gastrulation / genetics,  physiology*
Germ Cells / cytology*,  metabolism*
In Situ Hybridization
Grant Support
ID/Acronym/Agency:
R01 GM078341/GM/NIGMS NIH HHS; R01GM078341/GM/NIGMS NIH HHS; R21HD058953/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/Cadherins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Origin of Drosophila mushroom body neuroblasts and generation of divergent embryonic lineages.
Next Document:  Evolutionarily conserved requirement of Cdx for post-occipital tissue emergence.