Document Detail

Dystrophin disruption might be related to myocardial cell apoptosis caused by isoproterenol.
MedLine Citation:
PMID:  11206716     Owner:  NLM     Status:  MEDLINE    
Sarcolemma integrity is stabilized by the dystrophin-associated glycoprotein complex that connects actin and laminin-2 in contractile machinery and the extracellular matrix, respectively. Interruption of the connection by the primary gene defect or acquired pathological burden can cause cardiac failure. The purposes of the present study were to verify whether dystrophin is disrupted in acute myocardial injury after the isoproterenol overload (10 mg/kg) and to examine its relation to myocardial cell apoptosis in rats. This injury from 4-16 h at the subendocardium was accompanied by dystrophin disruption and dislocation from subsarcolemma to cytoplasm, which were confirmed by immunohistology and Western blotting. However, delta-sarcoglycan was thoroughly preserved in sarcolemma. The dystrophin degradation preceded the appearance of apoptotic cells and exactly coincided with the transferase-mediated dUTP-biotin nick end labeling-positive cardiomyocytes (TUNEL), as was verified by double-staining. These data suggest that beta-adrenergic stimulation induces dystrophin breakdown followed by apoptosis.
H Xi; W S Shin; J Suzuki; T Nakajima; T Kawada; Y Uehara; M Nakazawa; T Toyo-oka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  36 Suppl 2     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2000  
Date Detail:
Created Date:  2001-02-06     Completed Date:  2001-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S25-9     Citation Subset:  IM    
Department of Internal Medicine, Tokyo University Hospital, University of Tokyo, Japan.
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MeSH Terms
Adrenergic beta-Agonists / toxicity*
Apoptosis / drug effects*
Cytoskeletal Proteins / analysis
Dystrophin / analysis,  physiology*
Heart / drug effects*
Isoproterenol / toxicity*
Membrane Glycoproteins / analysis
Myocardium / pathology
Rats, Sprague-Dawley
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Cytoskeletal Proteins; 0/Dystrophin; 0/Membrane Glycoproteins; 146888-27-9/Dystroglycans; 7683-59-2/Isoproterenol

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