Document Detail


Dysregulated immunophenotypic attributes of plasmacytoid but not myeloid dendritic cells in HIV-1 infected individuals in the absence of highly active anti-retroviral therapy.
MedLine Citation:
PMID:  23039892     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cells (DC) in HIV-1-infected individuals are decreased and their dysfunction has been implicated in HIV-1 immunopathogenesis. The mechanism of their dysfunction remains unclear, thus we analysed the expression of membrane molecules associated with immune regulation and DC activation in myeloid (mDC) and plasmacytoid DC (pDC) in therapy-naive and highly active anti-retroviral therapy (HAART)-treated HIV-1(+) patients. DC from healthy controls, untreated HIV-1(+) and HAART-treated patients were assessed by flow cytometry for expression of: anergy and apoptosis inducing molecules [programmed death (PD)-1 and its ligands PD-L1 and PD-L2], inhibitory and regulatory T cell-inducing molecules [immunoglobulin-like transcript (ILT)-3 and ILT-4], interferon (IFN)-α inhibitory receptor (ILT-7) and co-stimulatory molecules (CD80, CD83, and CD86). pDC from untreated HIV-1(+) patients expressed significantly lower levels of ILT-7 compared to healthy controls, while HAART-treated patients showed normal expression. pDC were also found to express moderately higher levels of PD-L1 and ILT-3 and lower levels of PD-L2 receptors in untreated patients compared to controls and HAART-treated patients. No significant changes were observed in mDC. There were no associations between the percentages and levels of expression of these molecules by pDC and viral load or CD4 T cell count. In conclusion, pDC but not mDC from HIV-1(+) patients with active viraemia display higher levels of apoptosis and T regulatory-inducing molecules and may be predisposed to chronically produce IFN-α through down-regulation of ILT-7. HAART restored normal expression levels of these receptors.
Authors:
A Benlahrech; A Yasmin; S J Westrop; A Coleman; A Herasimtschuk; E Page; P Kelleher; F Gotch; N Imami; S Patterson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  170     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-03-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  212-21     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Clinical and Experimental Immunology © 2012 British Society for Immunology.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD / immunology,  metabolism
Antigens, CD274 / immunology,  metabolism
Antiretroviral Therapy, Highly Active / methods
Cohort Studies
Dendritic Cells / immunology*,  metabolism
Female
HIV Infections / drug therapy,  immunology*,  metabolism,  pathology
HIV-1 / immunology
Humans
Immunophenotyping
Male
Membrane Glycoproteins / immunology,  metabolism
Middle Aged
Myeloid Cells / immunology*,  metabolism
Programmed Cell Death 1 Receptor / immunology,  metabolism
Receptors, Cell Surface / immunology,  metabolism
Receptors, Immunologic / immunology,  metabolism
Viremia / immunology,  metabolism
Young Adult
Grant Support
ID/Acronym/Agency:
G0100482//Medical Research Council
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD274; 0/CD274 protein, human; 0/LILRA4 protein, human; 0/LILRB2 protein, human; 0/LILRB4 protein, human; 0/Membrane Glycoproteins; 0/PDCD1 protein, human; 0/Programmed Cell Death 1 Receptor; 0/Receptors, Cell Surface; 0/Receptors, Immunologic
Comments/Corrections

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