Document Detail

Dysfunctional γδ T cells contribute to impaired keratinocyte homeostasis in mouse models of obesity.
Jump to Full Text
MedLine Citation:
PMID:  21833015     Owner:  NLM     Status:  MEDLINE    
Skin complications and chronic non-healing wounds are common in obesity, metabolic disease, and type 2 diabetes. Epidermal γδ T cells normally produce keratinocyte growth factors, participate in wound repair, and are necessary for keratinocyte homeostasis. We have determined that in γδ T cell-deficient mice, there are reduced numbers of keratinocytes and the epidermis exhibits a flattened, thinner structure with fewer basal keratinocytes. This is important in obesity, where skin-resident γδ T cells are reduced and rendered dysfunctional. Similar to γδ T cell-deficient mice, keratinocytes are reduced and the epidermal structure is altered in two obese mouse models. Even in regions where γδ T cells are present, there are fewer keratinocytes in obese mice, indicating that dysfunctional γδ T cells are unable to regulate keratinocyte homeostasis. The impact of absent or impaired γδ T cells on epidermal structure is exacerbated in obesity as E-cadherin localization and expression are additionally altered. These studies reveal that γδ T cells are unable to regulate keratinocyte homeostasis in obesity and that the obese environment further impairs skin structure by altering cell-cell adhesion. Together, impaired keratinocyte homeostasis and epidermal barrier function through direct and indirect mechanisms result in susceptibility to skin complications, chronic wounds, and infection.
Kristen R Taylor; Anne E Costanzo; Julie M Jameson
Related Documents :
9160895 - Role of high molecular weight extracellular matrix proteins in glioma cell migration.
3540015 - Antineuron specific enolase staining reactions in sarcomas and carcinomas: its lack of ...
1327005 - Melanotic neuroectodermal tumour as a predominant component of an immature testicular t...
8045525 - Atypical carcinoid tumour of the lung: a study of 33 cases with prognostic features.
1612785 - Fibroblast-dependent tumorigenicity of melanoma xenografts in athymic mice.
2040025 - Cadmium-resistant chinese hamster v79 cells with decreased accumulation of cadmium.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-08-11
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  131     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-10     Completed Date:  2012-01-11     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2409-18     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cadherins / metabolism
Diabetes Mellitus, Type 2 / metabolism,  physiopathology
Disease Models, Animal
Homeostasis / physiology*
Keratinocytes / metabolism*
Mice, Inbred C57BL
Mice, Obese
Obesity / metabolism*,  physiopathology*
Receptors, Antigen, T-Cell, gamma-delta / metabolism
T-Lymphocyte Subsets / metabolism*
Grant Support
Reg. No./Substance:
0/Cadherins; 0/Receptors, Antigen, T-Cell, gamma-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-journal-id): 0426720
Journal ID (pubmed-jr-id): 4839
Journal ID (nlm-ta): J Invest Dermatol
ISSN: 0022-202X
ISSN: 1523-1747
Article Information
Download PDF

nihms-submitted publication date: Day: 15 Month: 7 Year: 2011
Electronic publication date: Day: 11 Month: 8 Year: 2011
Print publication date: Month: 12 Year: 2011
pmc-release publication date: Day: 1 Month: 6 Year: 2012
Volume: 131 Issue: 12
First Page: 2409 Last Page: 2418
ID: 3213272
PubMed Id: 21833015
DOI: 10.1038/jid.2011.241
ID: nihpa311101

Dysfunctional γδ T cells contribute to impaired keratinocyte homeostasis in mouse models of obesity
Kristen R. Taylor
Anne E. Costanzo
Julie M. Jameson
The Scripps Research Institute, Department of Immunology and Microbial Science, La Jolla, CA 92037, USA
Correspondence: Correspondence should be addressed to Dr. Julie M. Jameson, The Scripps Research Institute, Department of Immunology and Microbial Science, 10550 North Torrey Pines Road, IMM-25, La Jolla, CA 92037. Phone: 858-784-8157. Fax: 858-784-8179.


Skin pathologies are common in obesity, metabolic disease and type 2 diabetes. The underlying mechanisms behind these skin complications are not fully understood (Guida et al., 2010; Paron and Lambert, 2000; Sibbald et al., 1996). In obesity and metabolic disease, diminished or altered levels of growth factors occur at the wound site, impairing leukocyte infiltration and cell growth and migration over the wound (Blakytny and Jude, 2006). Despite intensive research over the years, there has been minimal progress in treatments targeted at resolving wound repair deficiencies in these patients. Several mouse models of obesity and metabolic disease have been investigated to better understand epidermal dysfunction in non-wounded and wounded tissue. Obese mice have mechanically weaker skin than lean mice (Enser and Avery, 1984), reduced expression of growth factors and growth factor receptors (Beer et al., 1997; Frank et al., 1995; Werner et al., 1994) and impaired insulin signaling in keratinocytes in non-wounded tissue (Goren et al., 2006). However, to our knowledge, little has been published on keratinocyte homeostasis and epidermal structure and function impairment in obesity even before damage or injury.

In addition to keratinocytes, a lymphocyte population of γδ T cells, also referred to as dendritic epidermal T cells (DETC), reside in the epidermis. These canonical Vγ3Vδ1 T cells (alternate nomenclature Vγ5Vδ1) maintain epithelial integrity and regulate homeostasis of keratinocytes through the production of growth factors (Girardi, 2006; Havran and Jameson, 2010). The absence of γδ T cells in the epidermis compromises the epithelial barrier, suggesting that γδ T cells provide local factors that facilitate epidermal homeostasis (Girardi et al., 2006; Sharp et al., 2005). We have recently determined that epidermal γδ T cells in obese mice have reduced numbers, altered proliferation and diminished growth factor production (Taylor et al., 2010). This suggests that obesity impairs the ability of epidermal T cells to perform regulatory functions in the epidermis even before injury. The influence of dysfunctional epidermal T cells on the homeostasis of neighboring cells in the obese environment is unknown.

Here we investigate the hypothesis that dysfunctional γδ T cells in obesity negatively impact keratinocyte homeostasis, altering barrier structure and function. Using mice deficient in γδ T cells (TCRδ−/− mice), we investigated keratinocyte homeostasis in the absence of γδ T cells and compared that to keratinocytes in two independent mouse models of obesity. Our studies reveal that keratinocytes are reduced and the epidermis has a flattened and thinner appearance in obese mice similar to TCRδ−/− mice, supporting our hypothesis that dysfunctional γδ T cells contribute to impaired keratinocyte homeostasis in obesity. In addition to dysfunctional γδ T cells, keratinocyte homeostasis is further exacerbated by factors in the obese environment as demonstrated by altered E-cadherin localization and impaired cell-cell adhesion. This influences epithelial integrity in obesity and would increase the susceptibility of the epidermis to damage, environmental insults and pathogens.

γδ T cells regulate keratinocyte numbers and epidermal structure

Mice deficient in γδ T cells (TCRδ−/−) provide an important tool for studying epidermal function in the absence of γδ T cells. It has been previously demonstrated that γδ T cells are necessary for keratinocyte survival (Sharp et al., 2005), but it is unknown how γδ T cells impact overall keratinocyte and epidermal homeostasis. As a replacement for γδ T cells in TCRδ−/− mice, αβ T cells seed and populate the epidermis (Jameson et al., 2004). However, these αβ T cells are unable to recognize and respond to keratinocyte damage (Jameson et al., 2004; Sharp et al., 2005). In TCRδ−/− mice we observed consistently fewer keratinocytes as compared to wild-type (WT) C57BL/6J (B6) mice as determined by immunofluorescent microscopy of epidermal sheets (Figure 1a).

We next determined whether keratinocytes require a closely-neighboring γδ T cell or if an αβ T cell can provide the necessary contact through adhesion molecule interactions, such as CD103. We examined keratinocyte numbers in epidermal sheets and found that in WT mice, areas with 2 γδ T cells had a higher number of keratinocytes (35.2 ± 2.4 keratinocytes/grid) than areas with 0 γδ T cells (28.0 ± 2.5 keratinocytes/grid) (Figure 1b). However, in TCRδ−/− mice with αβ T cells instead of γδ T cells, a reduced number of keratinocytes (30.9 ± 1.6 keratinocytes/grid) was observed in areas of 2 αβ T cells (Figure 1b). The presence of 2 αβ T cells resulted in similar keratinocyte numbers as an area in a WT mouse that was devoid of γδ T cells (28.0 vs. 30.9, respectively). This data demonstrates that the presence of closely-neighboring γδ T cells impacts keratinocyte homeostasis and that the keratinocyte-responsive TCR is necessary for this function.

To determine whether altered keratinocyte homeostasis impacts epidermal structure, we the epidermis of WT and TCRδ−/− mice was examined by immunofluorescence microscopy. The basal layer of proliferating keratinocytes is recognized by antibodies directed against keratin 5 (K5), a marker of keratinocyte proliferation, while antibodies against keratin 1 (K1) identify cells undergoing the early stages of differentiation. The epidermis in TCRδ−/− mice was thinner than WT mice and keratinocyte nuclei had a flattened morphology with fewer basal membrane keratinocytes (Figure 1c). In TCRδ−/− mice, regions of keratin 1 staining dipped down into the basal proliferating layer, suggesting altered keratinocyte proliferation and differentiation in the absence of γδ T cells. Western blot analysis demonstrated proportional keratin 5 and keratin 1 staining (data not shown). Therefore, the absence of γδ T cells results in reduced keratinocyte numbers, fewer keratinocytes along the basement membrane, a flattened and thinner epidermis and altered localization of keratin 1 staining.

Reduced keratinocyte numbers in obese mice

Keratinocytes require γδ T cells for homeostasis, however, we have previously demonstrated that skin γδ T cells are dysfunctional in obesity (Taylor et al., 2010). To investigate how dysfunctional γδ T cells impact keratinocyte homeostasis in obesity, two separate mouse models of disease were utilized, C57BLKS/J (BKS) db/db and B6 high fat diet (HFD) mice. Since a genetic mutation in the leptin receptor in BKS db/db mice results in severe obesity and metabolic syndrome, we compared two ages: young 6-week old db/db mice before the onset of disease and adult 10- to 14-week old obese db/db mice. This allowed us to determine that alterations observed in obese mice, but not 6-week old mice, were due to obesity and not genetic deficiency of the leptin receptor. Additionally, we employed a second mouse model of obesity, the B6 HFD model, where wild-type male B6 mice are fed a 60 kcal% fat diet. HFD mice have a slower progression of obesity and metabolic disease that more closely mimics human disease.

To determine overall keratinocyte numbers, we examined immunofluorescent images of epidermal sheets to quantify the number of keratinocytes per square millimeter (mm). A comparable number of keratinocytes were observed in 6-week old db/+ and db/db mice (Figure 2a). However, obese 10- and 14-week old db/db animals exhibited fewer keratinocytes/mm2 as their lean db/+ littermates (Figure 2a). No further reduction in keratinocyte numbers was observed in mice older than 14 weeks (Figure 2a). Similarly, we found diminished numbers of keratinocytes in the epidermis of obese HFD mice as compared to their lean normal chow diet (NCD) counterparts (Figure 2b). Thus, as obesity and related metabolic disease progresses, keratinocyte numbers become reduced.

Keratinocytes require functional γδ T cells for epidermal homeostasis

We next investigated whether keratinocytes in obese mice are reduced when neighboring γδ T cells are dysfunctional, similar to keratinocytes lacking neighboring γδ T cells in TCRδ−/− mice. To investigate the correlation of keratinocyte homeostasis and γδ T cells, we examined epidermal sheets from lean db/+ mice to quantify the number of keratinocytes and closely-neighboring γδ T cells. As shown in Figure 3a (upper panels), we found that areas with more γδ T cells (2 γδ T cells) correlated with a higher number of keratinocytes (37.3 ± 3.7 keratinocytes/grid). However, if γδ T cells were completely absent from the area (0 γδ T cells), then there were fewer keratinocytes (29.9 ± 4.4 keratinocytes/grid).

Next, we asked whether a γδ T cell needs to be functional to promote keratinocyte homeostasis. As shown in Figure 3a (lower panels), obese db/db mice still had reduced keratinocyte numbers (30.7 ± 3.4 keratinocytes/grid) even in areas with 2 γδ T cells. These areas resemble those of lean control mice with 0 γδ T cells. This demonstrates that although γδ T cells are present in obese animals, their inability to function properly ultimately impacts keratinocyte homeostasis. Further reduction of γδ T cells in obese db/db mice (0 γδ T cells) demonstrated more severe loss of keratinocytes (24.5 ± 2.8 keratinocytes/grid) (Figure 3a).

Additionally, keratinocyte numbers in lean B6 NCD mice demonstrated that areas with 2 γδ T cells have a higher number of keratinocytes (38.1 ± 3.4 keratinocytes/grid) than areas with 0 γδ T cells (28.7 ± 3.1 keratinocytes/grid) (Figure 3b, upper panels). Similar to obese db/db mice, keratinocytes were reduced in obese HFD mice (31.5 ± 3.3 keratinocytes/grid) even if 2 γδ T cell were present (Figure 3b, lower panels). Unlike in db/db mice, areas with 0 γδ T cells in the HFD mice only had a slight reduction in keratinocytes (29.9 ± 2.5 keratinocytes/grid) than areas of 2 γδ T cells, suggesting that the further reduction observed in db/db animals is likely due to the severity of metabolic disease or the contribution of genetic deficiency of the leptin receptor. Together, these data demonstrate that the presence alone of a resident γδ T cell proximal to a neighboring keratinocyte is not enough to maintain the proper homeostatic environment for the keratinocytes. In fact, the γδ T cell must be functional to perform this regulatory role.

Obese mice have a disorganized epidermis

Experiments using TCRδ−/− mice demonstrated that γδ T cells are important for keratinocyte homeostasis and epidermal structure. We performed immunofluorescent microscopy on frozen skin sections isolated from the db/db and HFD mouse models to determine whether the epidermis is altered in obesity. Consistent with wild-type animals, skin sections isolated from 6-week old db/+ and db/db mice display a similar epidermal thickness and normal nuclei staining (Figure 4a). However, the epidermis of 12-week old obese db/db and HFD sections had several structural differences when compared to lean db/+ and NCD epidermis, respectively (Figure 4b and c). The overall thickness of the epidermis in obese db/db and HFD sections was thinner, there were fewer keratinocytes along the basement membrane and the remaining basal keratinocytes had a more flattened appearance, similar to TCRδ−/− epidermis (Figure 1c).

In non-obese 6-week old db/db animals, keratin 5 was restricted to the basal keratinocytes and keratin 1 localized to the suprabasal layer (Figure 4a). However, similar to TCRδ−/− mice, we observed an alteration in the pattern of keratin staining and localization in obese mice. Epithelial disorganization was observed in obese skin as keratin 1 was observed in the basal layer, sometimes directly adjacent to the basement membrane in sections from obese db/db and HFD mice. Epidermal sections in which keratinocytes co-stained for both keratin 5 and keratin 1 (yellow) were also evident in obese mice, suggesting altered regulation of proliferation and differentiation in keratinocytes (Figure 4b and c).

To further examine whether there was a total change in the expression of keratin 5 and keratin 1 protein in the epidermis, we performed immunoblot analysis on whole epidermal cell lysates. No differences in keratin 5 and keratin 1 expression were observed in 6-week old db/db mice as compared to db/+ animals (Figure 5a). However, diminished amounts of keratin 5 and enhanced amounts of keratin 1 were reproducibly observed in the epidermis of obese 12-week old db/db mice and HFD animals (Figure 5a). Densitometry of each Western blot is representative of a minimum of three independent experiments. Keratin 5 expression was subtlety decreased an average of 1.4 for db/db mice and 1.6 for HFD, although these reproducible decreases were not statistically significant. Keratin 1 expression exhibited an average fold change increase of 1.8 for db/db mice (p=0.03) and 2.2 (p=0.03) for HFD mice.

Furthermore, keratinocytes isolated from 10-week old obese db/db treated with Bromodeoxyuridine (BrdU) in their drinking water mice displayed a decreased incorporation of BrdU, confirming a diminished rate of keratinocyte proliferation in obesity (Figure 5b). Together, these data demonstrate that the epidermis in obese mice displays a flattened and thinner morphology with aberrant keratin 1 localization in the basal layer, similar to the epidermis in TCRδ−/− mice. However, the epidermis in obese mice is more severe than TCRδ−/− mice as they exhibit changes in the expression of proliferation and differentiation markers, demonstrating that the obese environment further exacerbates keratinocyte dysfunction.

Keratinocytes in obesity exhibit altered localization and diminished E-cadherin expression

Chronic inflammation and type 2 diabetes, including elevated insulin and glucose, can lead to disruption of adherens junctions and ultimately organ dysfunction (Dejana et al., 2008; Masur et al., 2011). Adherens junctions are comprised of cadherins and these cell-cell interactions are critical for cell communication and adhesion to provide an intact epithelial barrier (Muller et al., 2008). To determine whether adherens junctions were disrupted in obese mice, we investigated expression and localization of the prominent keratinocyte component of adherens junctions, E-cadherin. Immunofluorescent microscopy demonstrated that E-cadherin staining was localized to the intercellular borders in epidermal sheets isolated from db/+ and db/db mice (Figure 6a). Organized, single rows of E-cadherin staining were observed between keratinocytes in epidermal sheets of lean db/+ and NCD mice (Figure 6a and b, single arrows), whereas E-cadherin staining was reduced and demonstrated a disorganized double row staining pattern in obese db/db and HFD mice (Figure 6a and b, double arrows). Quantification of the E-cadherin junction width confirmed that the cell-cell junctions in obese mice are wider; suggesting that adhesion between cells is compromised.

Western blot analysis on epidermal cell lysates isolated from obese db/db and HFD animals confirmed that expression of full-length E-cadherin was decreased in obese mice as compared to lean mice (Figure 6c). This demonstrates that E-cadherin may be undergoing altered regulation, such as decreased expression or increased shedding, in the epidermis of obese mice, impacting cell-cell adhesion. However, we found no change in E-cadherin expression or localization in TCRδ−/− mice (Supplemental Figure 1a and b), supporting that the obese environment further exacerbates keratinocyte dysfunction through changes in cell-cell adhesion which may contribute to the more severe phenotype observed in obese mice.


Skin complications, chronic non-healing wounds and increased skin infections are all problems associated with obesity and type 2 diabetes. We investigated impaired keratinocyte homeostasis and epidermal structure in obese mice and examined how the dysfunction of neighboring γδ T cells impacts keratinocyte function. Using TCRδ−/− mice as a tool to study keratinocytes in the absence of γδ T cells, we determined that keratinocytes require the presence of a closely-neighboring γδ T cell for appropriate homeostasis. Regions that have two or more γδ T cells have a greater number of keratinocytes than regions that have zero or one γδ T cell. It is known that γδ T cells provide growth factors for keratinocytes (Havran and Jameson, 2010; Sharp et al., 2005) and TCRδ−/− mice have altered epidermal integrity and increased rate of transepidermal water loss (Girardi et al., 2006). We demonstrated that in the epidermis of TCRδ−/− and obese mice, keratinocyte numbers are reduced and the epidermis exhibits a thinner and flattened structure, with fewer keratinocytes along the basement membrane. Even with a neighboring γδ T cell, keratinocytes in obese mice still have reduced numbers suggesting that dysfunctional γδ T cells are unable to provide the necessary factors and/or contacts to neighboring keratinocytes. γδ T cells are also important for epithelial maintenance in the intestine as TCRδ−/− mice have a reduction in epithelial cell turnover (Komano et al., 1995). It is interesting to note that resident αβ T cells that take the place of γδ T cells in TCRδ−/− mice are unable to fully compensate, demonstrating that an inherent component of γδ T cells is necessary for keratinocyte homeostasis.

Keratinocytes in obese mice are further impacted by the surrounding environment, including inflammatory factors, elevated glucose and insulin resistance, which do not contribute to further keratinocyte dysfunction in TCRδ−/− mice. Wound healing is disrupted in TCRδ−/− mice (Jameson et al., 2002), however chronic non-healing wounds are more severe in obese mice suggesting that the obese environment plays a more direct role. As it is unknown how impaired γδ T cell function contributes to dysfunctional keratinocyte homeostasis, it has been important to decipher the role of each of these in disease. In obese mice, we observe a further impact on severity of keratinocyte dysfunction as demonstrated by more severe changes in epidermal structure and the maintenance of keratinocyte-keratinocyte contact through E-cadherin interactions. A variety of acute and chronic disorders, including inflammation, sepsis and diabetes, can all lead to disruption of cadherins in endothelial cells, compromising cadherin integrity and leading to organ dysfunction (Dejana et al., 2008). Our data suggests that factors within the obese environment have a direct effect on keratinocyte differentiation and adhesion and further exacerbate impaired epithelial structure and integrity in obesity.

This work is directly relevant to understanding skin complications associated with obesity and metabolic disease in humans. In contrast to the murine epidermis, the human epidermis consists of a mixed αβ and γδ population (Bos et al., 1987; Clark et al., 2006; Dupuy et al., 1990; Ebert et al., 2006; Foster et al., 1990). Despite this difference, human αβ and γδ epidermal T cells exhibit many features similar to the murine population such as the ability to upregulate IGF-1 after stimulation (Toulon et al., 2009). αβ and γδ T cells isolated from acute human wounds produce IGF-1, however, cells isolated from chronic non-healing wounds do not, providing the first evidence that human skin resident T cells are necessary for proper wound healing (Toulon et al., 2009). To date, the contribution of epidermal-resident T cells and their impact on keratinocyte homeostasis to skin complications associated with obesity and metabolic disease is unknown and represents a novel therapeutic target. It is important to decipher the contribution of dysfunctional skin-resident T cells and the obese environment to better develop targets for improving epithelial cell homeostasis and alleviating chronic skin pathologies and non-healing wounds.

Materials and Methods

Heterozygous C57BLKS/J db/+ and C57BL/6J TCRδ−/− were purchased from The Jackson Laboratory (Bar Harbor, ME), C57BL/6J mice were purchased from The Scripps Research Institute (TSRI) Rodent Breeding Facility and all were housed and bred at TSRI. For high-fat diet experiments, mice were switched to a 60 kcal% diet (Research Diets, Brunswick, NJ) at 6 weeks of age and used in experiments after 16 to 20 weeks on the diet. Experiments on female db/db mice were performed between 6 and 14 weeks of age and male and female B6 δ−/− mice between 8 and 12 weeks of age. C57BLKS/J db/db mice past 20 weeks of age were euthanized due to severe health complications (Herberg, 2001). Mice were given access to food and water ad libitum and were housed in sanitized conditions. All animal work was approved by TSRI Institutional Animal Care and Use Committee (protocol 08-0057).

In vivo BrdU treatment and analysis

Mice were treated with BrdU in their drinking water, epidermal cells were isolated from the back and belly skin of mice. BrdU incorporation was detected by flow cytometry as previously described (Jameson et al., 2002; Jameson et al., 2004; Taylor et al., 2010).

Western blot analysis

Epidermal cells were isolated from the back and belly skin of mice, immediately lysed and protein content was determined by Pierce BCA assay (Thermo Scientific, Waltham, MA). Immunoblots were analyzed using primary antibodies against E-cadherin (Cell Signaling, Danvers, MA), keratin 1 or keratin 5 (provided by Dr. Colin Jamora, University of California, San Diego), probed with secondary IgG-HRP antibodies (Southern Biotech, Birmingham, AL) and developed with Super Signal West Pico Chemiluminescence Kit (Thermo Scientific). Densitometry was determined using ImageJ software (NIH).

Immunofluorescent microscopy

Whole skin tissue was isolated from the upper back of the mice and was embedded midline down in O.C.T. compound (Tissue-Tek, Sakura Finetek USA, Inc., Torrance, CA) and cut into 10μm sections (Leica Cryostat). Sections were fixed with 4% methanol-free formaldehyde (Sigma-Aldrich) and immunostained with keratin 1 (1:250) or keratin 5 (1:400). Sections were probed with anti-rabbit FITC (1:100) or anti-chicken PE (1:100) (JacksonImmuno Research) secondary antibodies and mounted with SlowFade Gold Antifade medium (Invitrogen).

Epidermal sheets were isolated from ear sheet halves as described previously (Jameson et al., 2002; Jameson et al., 2004), fixed with ice-cold acetone and stained with antibodies directed against E-cadherin (or IgG isotype control), γδ TCR and CD45.2 (Biolegend, San Diego, CA). DAPI was used to counterstain. Digital images were acquired (Zeiss AzioCam HRc) and analyzed using Photoshop CS4 software (Adobe). For correlation of keratinocytes and γδ T cells, one square inch grids were outlined and the number of cells was quantified. Junction width was determined using the Photoshop software measure tool. A minimum of three sets of mice were used per experiment, with a minimum of 20 images acquired. Between 17 and 62 areas were counted for each experiment.


FN2Conflict of Interest

The authors declare no conflict of interest.

We gratefully acknowledge Lauren Aarreberg and Christa Park for technical assistance. We thank Pedro Lee and Dr. Colin Jamora at The University of California, San Diego for reagents and helpful advice. We would also like to thank Drs. Deborah Witherden, Amanda MacLeod and Wendy Havran at The Scripps Research Institute for helpful suggestions regarding experiments and data interpretation. This work is supported by National Institutes of Health grant DK073098 and DK080048 (JMJ) and a Department of Immunology and Microbial Science Institutional Training Grant 5T32 AI007244-24 (KRT). This is manuscript number 21098 from The Scripps Research Institute.

B6 C57BL/6J
HFD high fat diet
NCD normal chow diet
wks weeks
wk week
k5 keratin 5
k1 keratin 1
WT wild-type
IGF-1 insulin-like growth factor-1
KGF-1 keratinocyte growth factor-1

Beer HD,Longaker MT,Werner S. Year: 1997Reduced expression of PDGF and PDGF receptors during impaired wound healingJ Invest Dermatol10913289242497
Blakytny R,Jude E. Year: 2006The molecular biology of chronic wounds and delayed healing in diabetesDiabet Med2359460816759300
Bos JD,Zonneveld I,Das PK,et al. Year: 1987The skin immune system (SIS): distribution and immunophenotype of lymphocyte subpopulations in normal human skinJ Invest Dermatol88569733494791
Clark RA,Chong B,Mirchandani N,et al. Year: 2006The vast majority of CLA+ T cells are resident in normal skinJ Immunol1764431916547281
Dejana E,Orsenigo F,Lampugnani MG. Year: 2008The role of adherens junctions and VE-cadherin in the control of vascular permeabilityJ Cell Sci12121152218565824
Dupuy P,Heslan M,Fraitag S,et al. Year: 1990T-cell receptor-γ/δ bearing lymphocytes in normal and inflammatory human skinJ Invest Dermatol9476482141342
Ebert LM,Meuter S,Moser B. Year: 2006Homing and function of human skin γδ T cells and NK cells: relevance for tumor surveillanceJ Immunol1764331616547270
Enser M,Avery NC. Year: 1984Mechanical and chemical properties of the skin and its collagen from lean and obese-hyperglycaemic (ob/ob) miceDiabetologia274496468798
Foster CA,Yokozeki H,Rappersberger K,et al. Year: 1990Human epidermal T cells predominantly belong to the lineage expressing α/β T cell receptorJ Exp Med17199710132182763
Frank S,Hubner G,Breier G,et al. Year: 1995Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healingJ Biol Chem27012607137759509
Girardi M. Year: 2006Immunosurveillance and immunoregulation by γδ T cellsJ Invest Dermatol126253116417214
Girardi M,Lewis JM,Filler RB,et al. Year: 2006Environmentally responsive and reversible regulation of epidermal barrier function by γδ T cellsJ Invest Dermatol1268081416439970
Goren I,Muller E,Pfeilschifter J,et al. Year: 2006Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-αAm J Pathol1687657716507892
Guida B,Nino M,Perrino NR,et al. Year: 2010The impact of obesity on skin disease and epidermal permeability barrier statusJ Eur Acad Dermatol Venereol24191519929936
Havran WL,Jameson JM. Year: 2010Epidermal T cells and wound healingJ Immunol1845423820483798
Herberg L,Leiter EH. Sima A,Shafrir EYear: 2001Obesity/diabetes in mice with mutations in the leptin or leptin receptor genesAnimal Models of Diabetes: A PrimerAmsterdamHarwood Academic Publishers63107
Jameson J,Ugarte K,Chen N,et al. Year: 2002A role for skin γδ T cells in wound repairScience296747911976459
Jameson JM,Cauvi G,Witherden DA,et al. Year: 2004A keratinocyte-responsive γδ TCR is necessary for dendritic epidermal T cell activation by damaged keratinocytes and maintenance in the epidermisJ Immunol1723573915004158
Komano H,Fujiura Y,Kawaguchi M,et al. Year: 1995Homeostatic regulation of intestinal epithelia by intraepithelial γδ T cellsProc Natl Acad Sci U S A926147517597094
Masur K,Vetter C,Hinz A,et al. Year: 2011Diabetogenic glucose and insulin concentrations modulate transcriptome and protein levels involved in tumour cell migration, adhesion and proliferationBr J Cancer1043455221179032
Muller EJ,Williamson L,Kolly C,et al. Year: 2008Outside-in signaling through integrins and cadherins: a central mechanism to control epidermal growth and differentiation?J Invest Dermatol1285011618268536
Paron NG,Lambert PW. Year: 2000Cutaneous manifestations of diabetes mellitusPrim Care273718310815049
Sharp LL,Jameson JM,Cauvi G,et al. Year: 2005Dendritic epidermal T cells regulate skin homeostasis through local production of insulin-like growth factor 1Nat Immunol673915592472
Sibbald RG,Landolt SJ,Toth D. Year: 1996Skin and diabetesEndocrinol Metab Clin North Am25463728799710
Taylor KR,Mills RE,Costanzo AE,et al. Year: 2010γδ T Cells Are Reduced and Rendered Unresponsive by Hyperglycemia and Chronic TNFalpha in Mouse Models of Obesity and Metabolic DiseasePLoS One5e1142220625397
Toulon A,Breton L,Taylor KR,et al. Year: 2009A role for human skin-resident T cells in wound healingJ Exp Med2067435019307328
Werner S,Breeden M,Hubner G,et al. Year: 1994Induction of keratinocyte growth factor expression is reduced and delayed during wound healing in the genetically diabetic mouseJ Invest Dermatol103469737930669

Article Categories:
  • Article

Previous Document:  Establishment of murine basal cell carcinoma allografts: a potential model for preclinical drug test...
Next Document:  Generation of melanocytes from induced pluripotent stem cells.