Document Detail


Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder.
MedLine Citation:
PMID:  19885620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single-prolonged stress (SPS) is an established animal model for post-traumatic stress disorder (PTSD). The calcium (Ca2+)-calmodulin (CaM)-CaM kinase (K)IIalpha signal passage plays an important role in the plasticity of central nervous, learning and memory, mind and behavior and other types of cognitive activities. The amygdala is known to play an important role in fear, rage and emotional memory. In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly increased, and CaMKIIalpha expression significantly decreased (P<0.05) in the basolateral amygdala after SPS. These findings suggest dysfunction of Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of SPS rats, which may play important roles in the pathogenesis of PTSD rats.
Authors:
Bing Xiao; Fang Han; Yu-Xiu Shi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  24     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-03     Completed Date:  2010-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  795-9     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, Basic Medical Sciences College, China Medical University, Shenyang 110001, PR China.
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MeSH Terms
Descriptor/Qualifier:
Amygdala / enzymology*,  pathology
Animals
Blotting, Western
Calcium / metabolism
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics,  metabolism*
Calmodulin / genetics,  metabolism
Immunohistochemistry
Intracellular Fluid / chemistry
Male
Rats
Rats, Wistar
Stress Disorders, Post-Traumatic / enzymology*
Stress, Physiological / physiology
Stress, Psychological / metabolism
Chemical
Reg. No./Substance:
0/Calmodulin; 7440-70-2/Calcium; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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