Document Detail

Dysfunction in Cytochrome c Oxidase Caused by Excessive Nitric Oxide in Human Lens Epithelial Cells Stimulated with Interferon-γ and Lipopolysaccharide.
MedLine Citation:
PMID:  22632269     Owner:  NLM     Status:  Publisher    
Purpose: We previously found two mechanisms for the dysfunction in Ca(2+) regulation caused by excessive nitric oxide (NO) using the lenses of hereditary cataract model rats: the first is that NO causes a decrease in Adenosine-5'-triphosphate (ATP) level via cytochrome c oxidase (CCO), resulting in a decrease in ATPase function; the second is that NO causes enhanced lipid peroxidation, resulting in the oxidative inhibition of Ca(2+)-ATPase. In this study, we demonstrate the effect of excessive NO on lipid peroxidation and ATP production in human lens using a human lens epithelial cell line, SRA 01/04 (human lens epithelial (HLE) cells). Methods: Excessive NO via inducible NO synthase (iNOS) was induced by stimulating cells with a combination of interferon-gamma (1000 IU IFN-γ) and lipopolysaccharide (100 ng/mL LPS). CCO activity was measured using a Mitochondrial Isolation kit and Cytochrome c Oxidase Assay kit, and ATP levels were determined using a Sigma ATP Bioluminescent Assay Kit and a luminometer AB-2200. Results: Cytochrome c oxidase activity and ATP levels were decreased in HLE cells stimulated with IFN-γ and LPS, and aminoguanidine (AG) and diethyldithiocarbamate (DDC) added 6 h before cell collection significantly attenuated these decreases in cells stimulated with the IFN-γ and LPS for 24-30 h. However, the lower CCO activity and ATP levels in HLE cells stimulated with the IFN-γ and LPS for 30 h were not changed by treatment with AG or DDC for 6-12 h, while the CCO activity and ATP levels in HLE cells treated with AG or DDC for 18 were recovered. Conclusion: Excessive NO causes a decrease in CCO activity and ATP levels, and the recovery time for CCO activity is related to exposure time to NO in HLE cells.
Noriaki Nagai; Yoshimasa Ito
Related Documents :
7661219 - Multinucleate cell angiohistiocytoma of the orbit.
21813729 - The impact of ovulation on fallopian tube epithelial cells: evaluating three hypotheses...
21641729 - Ovarian enzymatically active stromal cells can be a promoter of ovarian surface epithel...
8238729 - Atypical fibroxanthoma. multiple immunohistologic profiles.
2763379 - Ultrastructural behavior of human immunodeficiency virus (hiv) in multinucleated giant ...
479429 - Giant cells in male pattern alopecia: a histologic marker and pathogenetic clue.
7928949 - Glucose upshift of carbon-starved marine vibrio sp. strain s14 causes amino acid starva...
17428869 - Construction and characterization of a full-length infectious simian t-cell lymphotropi...
2710689 - Aberrant response in vitro of hormone-responsive prostate cancer cells to antiandrogens.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-25
Journal Detail:
Title:  Current eye research     Volume:  -     ISSN:  1460-2202     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
School of Pharmacy and Pharmaceutical Research and Technology Institute, Kinki University , Higashi-Osaka, Osaka , Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Intramolecular Impact to the Sequence Specificity of B?A Transition: Low Energy Conformational V...
Next Document:  Disseminated tumour cells in bone marrow of patients with uveal melanoma.