Document Detail


Dynein-2 and ciliogenesis in Tetrahymena.
MedLine Citation:
PMID:  19562737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dynein-2 is the motor responsible for retrograde intraflagellar transport. In situ, dynein-2 comprises four subunits: the dynein-2 heavy chain (DYH2); the dynein-2 intermediate chain; the dynein-2 light-intermediate chain (D2LIC); and dynein light chain 8 (Rompolas et al. 2007. Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor. J Cell Sci 120:3653-3665). In contrast to what has been reported in other model organisms, when the DYH2 gene or the D2LIC gene was disrupted in Tetrahymena, the cells continued to produce motile cilia that were not swollen or filled with material [Rajagopalan et al.2009. Dynein-2 affects the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena thermophila. Mol Biol Cell 20:708-720]. When compared to wildtype cells, the dynein-2 mutants were found to have cilia that were at a lower density, shorter, and much more variable in length. One possible explanation for these effects is that the dynein-2 knockout cells grow cilia too slowly to enable them to achieve normal length and density before the cell divides. In the present study, dynein-2 knockout cells were deciliated and then allowed to regrow their cilia for 22 hr under conditions in which the cells did not divide. When dynein-2 was disabled, three effects were observed: (1) a decreased rate of cilia growth; (2) a lower cilia density that did not change over time; and (3) a wide distribution of cilia lengths that increased over time. These results confirm the importance of dynein-2 in regulating ciliary length in Tetrahymena. Cell Motil. Cytoskeleton, 2009. (c) 2009 Wiley-Liss, Inc.
Authors:
David J Asai; Vidyalakshmi Rajagopalan; David E Wilkes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  66     ISSN:  1097-0169     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-16     Completed Date:  2009-10-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  673-7     Citation Subset:  IM    
Affiliation:
Department of Biology, Harvey Mudd College, Claremont, California, USA. asaid@hhmi.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Cilia / metabolism*
Dyneins / genetics,  physiology*
Microscopy, Confocal
Microscopy, Fluorescence
Mutation
Protozoan Proteins / genetics,  physiology*
Tetrahymena / cytology*,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Protozoan Proteins; EC 3.6.4.2/Dyneins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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