| Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 16968746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Saccharomyces cerevisiae contains three dynamin-related-proteins, Vps1p, Dnm1p and Mgm1p. Previous data from glucose-grown VPS1 and DNM1 null mutants suggested that Vps1p, but not Dnm1p, plays a role in regulating peroxisome abundance. Here we show that deletion of DNM1 also results in reduction of peroxisome numbers. This was not observed in glucose-grown dnm1 cells, but was evident in cells grown in the presence of oleate. Similar observations were made in cells lacking Fis1p, a protein involved in Dnm1p function. Fluorescence microscopy of cells producing Dnm1-GFP or GFP-Fis1p demonstrated that both proteins had a dual localization on mitochondria and peroxisomes. Quantitative analysis revealed a greater reduction in peroxisome number in oleate-induced vps1 cells relative to dnm1 or fis1 cells. A significant fraction of oleate-induced vps1 cells still contained two or more peroxisomes. Conversely, almost all cells of a dnm1 vps1 double-deletion strain contained only one, enlarged peroxisome. This suggests that deletion of DNM1 reinforces the vps1 peroxisome phenotype. Time-lapse imaging indicated that during budding of dnm1 vps1 cells, the single peroxisome present in the mother cell formed long protrusions into the developing bud. This organelle divided at a very late stage of the budding process, possibly during cytokinesis. |
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Authors:
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Kasinath Kuravi; Shirisha Nagotu; Arjen M Krikken; Klaas Sjollema; Markus Deckers; Ralf Erdmann; Marten Veenhuis; Ida J van der Klei |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-12 |
Journal Detail:
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Title: Journal of cell science Volume: 119 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-21 Completed Date: 2007-01-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 3994-4001 Citation Subset: IM |
Affiliation:
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Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, PO Box 14, NL-9750 AA Haren, The Netherlands. |
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| MeSH Terms | |
Descriptor/Qualifier:
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GTP Phosphohydrolases
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genetics,
physiology* GTP-Binding Proteins / physiology* Mitochondria / metabolism Mitochondrial Proteins / genetics Models, Biological Oleic Acid / pharmacology Organelles / physiology Organisms, Genetically Modified Peroxisomes / metabolism* Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / genetics, physiology* Tissue Distribution Vesicular Transport Proteins |
| Chemical | |
Reg. No./Substance:
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0/Fis1p protein, S cerevisiae; 0/Mitochondrial Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Vesicular Transport Proteins; 112-80-1/Oleic Acid; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.1.-/VPS1 protein, S cerevisiae; EC 3.6.5.5/DNM1 protein, S cerevisiae |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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