Document Detail


Dynamics of viral load in plasma and HIV DNA in lymphocytes during highly active antiretroviral therapy (HAART): high viral burden in macrophages after 1 year of treatment.
MedLine Citation:
PMID:  11330367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we evaluated the level of HIV RNA in plasma and HIV DNA in peripheral blood cells. Sixteen antiretroviral-experienced HIV patients with severe immune suppression were included in the study. After the first month, 56.2% of the patients showed undetectable levels of HIV RNA, this percentage remaining stable after 1 year (53.3%). At enrollment, 7 patients (43.7%) with a low CD4+ T cell count (mean 22 per mm3 versus 73) showed HIV DNA levels below the limit of detection (5 copies/10(5)) in lymphocytes. They all subsequently had increased HIV DNA that became detectable between the first and the third month of therapy, associated with an increase of the CD4+ T cell count (mean 22 to 95/mm3); in 4 of these patients this increase was transitory, becoming undetectable again after one year. In 7 out of the 8 patients with detectable HIV DNA at enrollment, the HIV DNA level decreased over time. Seven out of 15 patients at 1 year (46.7%) showed both undetectable levels of HIV RNA in plasma and HIV DNA in lymphocytes (p<0.05); these patients had a higher CD4+ T cell count at baseline (mean 75 versus 25/mm3) and a higher increase (306 versus 177/mm3) after 1 year. PCR-based dilution assay carried out at 1 year showed that all patients had a consistent amount of HIV DNA positive- CD4+ T lymphocytes and macrophages, with higher values in these last cells. The data indicate that a durable reservoir of virus is still present in both lymphocytes and monocytes, even after long-lasting HAART treatment.
Authors:
A R Garbuglia; M Zaccarelli; S Calcaterra; G Cappiello; R Marini; A Benedetto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chemotherapy (Florence, Italy)     Volume:  13     ISSN:  1120-009X     ISO Abbreviation:  J Chemother     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-09-06     Revised Date:  2009-08-04    
Medline Journal Info:
Nlm Unique ID:  8907348     Medline TA:  J Chemother     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  188-94     Citation Subset:  IM    
Affiliation:
Center of Virology, Spallanzani Hospital, IRCCS, Rome, Italy. argarbuglia@iol.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Antiretroviral Therapy, Highly Active*
CD4 Lymphocyte Count
DNA, Viral / analysis*
Disease Reservoirs*
Female
Follow-Up Studies
HIV Infections / drug therapy*,  immunology,  virology
Humans
Lymphocytes / virology
Macrophages / immunology,  virology*
Male
Polymerase Chain Reaction
RNA, Viral / analysis
Viral Load
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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