Document Detail


Dynamics of native β-actin mRNA transport in the cytoplasm.
MedLine Citation:
PMID:  21518164     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transport of messenger RNAs (mRNAs) in the cytoplasm is essential for localization to translation sites and for post-transcriptional regulation. Utilizing single-RNA sensitive probes and real-time fluorescence microscopy, we accurately quantified the dynamics of native, non-engineered, β-actin mRNAs within the cytoplasm of epithelial cells and fibroblasts for the first time. Using single-particle tracking and temporal analysis, we determined that native β-actin mRNAs, under physiologic conditions, exhibit bursts of intermittent, processive motion on microtubules, interspersed between time periods of diffusive motion, characterized by non-thermal enhanced diffusivity. When transport processes were perturbed via ATP depletion, temperature reduction, dynamitin overexpression and chemical inhibitors, processive motion was diminished or eliminated and diffusivity was reduced. These data support a model whereby processive, motor-driven motion is responsible for long-distance mRNA transport.
Authors:
Aaron W Lifland; Chiara Zurla; Joana Yu; Philip J Santangelo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-18
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  12     ISSN:  1600-0854     ISO Abbreviation:  Traffic     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-11     Completed Date:  2011-12-15     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1000-11     Citation Subset:  IM    
Copyright Information:
© 2011 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics,  metabolism*
Adenosine Triphosphate / metabolism
Cytoplasm / metabolism*
Epithelial Cells / metabolism,  physiology
Fibroblasts / metabolism,  physiology
Humans
Microscopy, Fluorescence / methods
Microtubule-Associated Proteins / metabolism
Microtubules / metabolism
RNA Processing, Post-Transcriptional / physiology*
RNA Transport
RNA, Messenger / genetics,  metabolism*
Temperature
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
EB009455/EB/NIBIB NIH HHS; GM094198/GM/NIGMS NIH HHS; R01 GM094198/GM/NIGMS NIH HHS; R01 GM094198-01/GM/NIGMS NIH HHS; R21 EB009455/EB/NIBIB NIH HHS; R21 EB009455-01A2/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Microtubule-Associated Proteins; 0/RNA, Messenger; 144198-36-7/dynactin; 8L70Q75FXE/Adenosine Triphosphate
Comments/Corrections

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