Document Detail


Dynamics of intracellular calcium in cultured human keratinocytes after localized cell damage.
MedLine Citation:
PMID:  23614748     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Recovery of cultured keratinocytes after scratch damage is considered to be a wound-healing model. In this study, we observed changes in intracellular calcium concentration ([Ca(2+) ]i ) in cultured human keratinocytes after scratch damage. Immediately after scratch damage, a wave of increased [Ca(2+) ]i radiated outward from the damaged area and then disappeared gradually. But, [Ca(2+) ]i remained elevated in a peripheral layer of cells around the damaged area for several minutes. This layer did not appear in calcium-free medium. When the culture was switched to calcium-free medium for 30 min immediately after scratch damage, then switched back to standard (Ca(2+) -containing) medium, the recovery ratio after 24 h was approximately 25% lower than that of the culture in standard medium throughout. We speculate that delineation of damage sites by a layer of cells with increased [Ca(2+) ]i might be part of a signalling pathway that appropriately directs the wound-healing process in epidermis.
Authors:
Moe Tsutsumi; Makiko Goto; Mitsuhiro Denda
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Publication Detail:
Type:  Letter    
Journal Detail:
Title:  Experimental dermatology     Volume:  22     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  367-9     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons A/S.
Affiliation:
Shiseido Research Center, Yokohama, Japan; Japan Science and Technology Agency, CREST, Tokyo, Japan.
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