Document Detail


Dynamics of interphase microtubules in Schizosaccharomyces pombe.
MedLine Citation:
PMID:  10898975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Microtubules in interphase Schizosaccharomyces pombe are essential for maintaining the linear growth habit of these cells. The dynamics of assembly and disassembly of these microtubules are so far uncharacterised. RESULTS: Live cell confocal imaging of alpha1 tubulin tagged with enhanced green fluorescent protein revealed longitudinally oriented, dynamically unstable interphase microtubule assemblies (IMAs). The IMAs were uniformly bright along their length apart from a zone of approximately doubly intense fluorescence commonly present close to their centres. The ends of each IMA switched from growth ( approximately 3.0 microm/min) to shrinkage ( approximately 4.5 microm/min) at 1.0 events per minute and from shrinkage to growth at 1.9 events per minute, and the two ends were equivalently dynamic, suggesting equivalent structure. We accordingly propose a symmetrical model for microtubule packing within the IMAs, in which microtubules are plus ends out and overlap close to the equator of the cell. IMAs may contain multiple copies of this motif; if so, then within each IMA end, the microtubule ends must synchronise catastrophe and rescue. When both ends of an IMA lodge in the hemispherical cell ends, the IMAs start to bend under compression and their overall growth rate is inhibited about twofold. Similar microtubule dynamics were observed in cells ranging in size from half to twice normal length. Patterned photobleaching indicated no detectable treadmilling or microtubule sliding during interphase. CONCLUSIONS: The consequence of the mechanisms described is continuous recruitment of microtubule ends to the ends of growing cells, supporting microtubule-based transport into the cell ends and qualitatively accounting for the essential role for microtubules in directing linear cell growth in S. pombe.
Authors:
D R Drummond; R A Cross
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current biology : CB     Volume:  10     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-10-03     Completed Date:  2000-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  766-75     Citation Subset:  IM    
Affiliation:
Molecular Motors Group, Marie Curie Research Institute, The Chart, Surrey, UK.
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MeSH Terms
Descriptor/Qualifier:
Green Fluorescent Proteins
Interphase*
Luminescent Proteins / genetics,  metabolism
Microscopy, Fluorescence
Microtubules / metabolism*
Mitosis
Recombinant Fusion Proteins / genetics,  metabolism
Schizosaccharomyces / cytology,  genetics,  metabolism*
Tubulin / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 0/Tubulin; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections
Comment In:
Curr Biol. 2001 Aug 7;11(15):R600-2   [PMID:  11516965 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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