Document Detail


Dynamics of human myocardial progenitor cell populations in the neonatal period.
MedLine Citation:
PMID:  18805183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Pluripotent cardiac progenitor cells resident in myocardium offer a potentially promising role in promoting recovery from injury. In pediatric congenital heart disease (CHD) patients, manipulation of resident progenitor cells may provide important new approaches to improving outcomes. Our study goals were to identify and quantitate populations of progenitor cells in human neonatal myocardium during the early postnatal period and determine the proliferative capacity of differentiated cardiac myocytes. METHODS: Immunologic markers of cell lineage (stage-specific embryonic antigen 4 [SSEA-4], islet cell antigen 1 [Isl1], c-kit, Nkx2.5, sarcoplasmic reticulum calcium-regulated ATPase type 2 [SERCA2]) and proliferation (Ki67) were localized in right ventricular biopsies from 32 CHD patients aged 2 to 93 days. RESULTS: Neonatal myocardium contains progenitor cells and transitional cells expressing progenitor and differentiated myocyte marker proteins. Some cells expressed the pluripotent cell marker c-kit and also coexpressed the myocyte marker SERCA2. Multipotent progenitor cells, identified by the expression of Isl1, were found. Ki67 was expressed in some myocytes and in nonmyocyte cells. A few cells expressing SSEA-4 and Isl1 were observed during the early postnatal period. Cells expressing c-kit, the premyocyte marker Nkx2.5, and Ki67 were found throughout the first postnatal month. A progressive decline in cell density during the first postnatal month was observed for c-kit+ cells (p = 0.0013) and Nkx2.5+ cells (p = 0.0001). The percentage of cells expressing Ki67 declined during the first 3 postnatal months (p = 0.0030). CONCLUSIONS: Cells in an incomplete state of cardiomyocyte differentiation continue to reside in the infant heart. However, the relative density of progenitor cells declines during the first postnatal month.
Authors:
Gabriel Amir; Xiaoyuan Ma; V Mohan Reddy; Frank L Hanley; Olaf Reinhartz; Chandra Ramamoorthy; R Kirk Riemer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  86     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-10-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1311-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Cardiothoracic Surgery, Pediatric Division, Stanford University School of Medicine, Stanford, California 94305-5407, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Cardiac Surgical Procedures
Cell Proliferation*
Cells, Cultured
Female
Heart Defects, Congenital / diagnosis,  surgery
Humans
Immunohistochemistry
Infant
Infant, Newborn
Male
Myocytes, Cardiac / pathology,  physiology*
Probability
Proto-Oncogene Proteins c-kit / metabolism,  physiology*
Regeneration / physiology*
Sensitivity and Specificity
Stem Cells / pathology,  physiology*
Chemical
Reg. No./Substance:
EC 2.7.10.1/Proto-Oncogene Proteins c-kit
Comments/Corrections
Comment In:
Ann Thorac Surg. 2008 Oct;86(4):1320   [PMID:  18805184 ]

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