Document Detail

Dynamics of heat shock protein 70 concentrations in peripheral blood lymphocyte lysates during pregnancy in lactating Holstein-Friesian cows.
MedLine Citation:
PMID:  19729195     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to characterize the dynamics of the concentrations of heat shock protein 70 kDa (HSP70) in peripheral blood lymphocytes of lactating Holstein-Friesian dairy cows (Bos taurus) during pregnancy. The detection of pregnancy was carried out and blood samples collected on Days 40, 90, 120, 150, 180, and 210 of gestation from 46 cows (11 primiparous and 35 pluriparous, 34 seropositive and 12 seronegative to Neospora caninum). Peripheral blood lymphocytes were isolated by density gradient centrifugation. Serologic analysis of Neospora infection and determinations of HSP70 concentrations in lymphocyte lysates were carried out using commercial enzyme-linked immunosorbent assay kits. Climate variables were monitored using on-farm data loggers. Heat shock protein 70 concentrations increased in lymphocytes as gestation progressed, particularly in primiparous cows, with no effect from Neospora infection, climate variables, milk production, semen-providing bull, or outcome of gestation (singletons or twins). Our results show that HSP70 concentrations increased in lymphocytes as gestation progressed and were not affected by stressful factors, such as milk production, heat stress, chronic infection (neosporosis), or twin pregnancies.
J L Y??niz; F L??pez-Gatius; S Almer??a; T Carretero; I Garc??a-Ispierto; B Serrano; R F Smith; H Dobson; P Santolaria
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-02
Journal Detail:
Title:  Theriogenology     Volume:  72     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2010-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1041-6     Citation Subset:  IM    
Institute of Environmental Sciences (IUCA), Department of Animal Production, University of Zaragoza, Huesca, Spain.
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MeSH Terms
Cattle / blood*
Centrifugation, Density Gradient
HSP70 Heat-Shock Proteins / blood*
Lactation / blood*
Linear Models
Lymphocytes / metabolism*
Pregnancy, Animal / blood*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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