| Dynamics of Non-conventional Intraepithelial Lymphocytes-NK, NKT, and γδ T-in Celiac Disease: Relationship with Age, Diet, and Histopathology. | |
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MedLine Citation:
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PMID: 21221796 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Intraepithelial lymphocytes (IEL) are a heterogeneous population of lymphocytes raised in celiac disease (CD), whose role in CD pathogenesis remains to be defined. AIMS: To investigate how the age of diagnosis, diet, and the severity of the histological lesions are related to the changes observed in unconventional IEL populations. METHODS: Prospective analysis of 101 confirmed celiac patients from a single center, including 66 at diagnosis (45 children, 21 adults) and 112 non-celiac controls (12 children, 100 adults). IEL from duodenal biopsies were studied by six-color flow cytometry. The results were analyzed in relationship with age, diet (gluten intake), and histopathology (Marsh type). RESULTS: In comparison with respective age controls, both children and adult patients showed duodenal intraepithelial lymphocytosis with significant differences in every single non-conventional IEL population: CD3+ TCR γδ, NK (CD3-, CD16+, CD56+), NKT (CD3+, CD161+, CD56+), and iNKT (CD3+ Vα24) (P < 0.001 for all). Gluten intake was not only directly associated with severe atrophy, but also with decreased percentages of NK (P = 0.02), NKT (P = 0.003), and iNKT (P = 0.03). Changes in iNKT and γδ IEL were more marked in celiac children compared with celiac adults (P = 0.02 and 0.01, respectively). In contrast, increased CD3+ TCR γδ were diet- and Marsh grade-independent. CONCLUSIONS: The typical phenotypical profile of intraepithelial lymphocytosis in untreated pediatric and adult celiacs consists of increased CD3+ TCR γδ populations with decreased NK, NKT, and iNKT cells. NK, NKT, and iNKT IEL, but not γδ IEL, are dynamic populations associated with diet, age, and histopathology. |
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Authors:
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Sara Calleja; Santiago Vivas; María Santiuste; Laura Arias; Mercedes Hernando; Esther Nistal; Javier Casqueiro; Jose G Ruiz de Morales |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-8 |
Journal Detail:
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Title: Digestive diseases and sciences Volume: - ISSN: 1573-2568 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Immunology, Complejo Hospitalario Universitario de León, Altos de Nava s/n, 24080, León, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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