Document Detail


Dynamics of GPI-anchored proteins on the surface of living cells.
MedLine Citation:
PMID:  17292110     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rather than being distributed homogeneously on the cell surface, proteins are probably aggregated in clusters or in specific domains. Some of these domains (lipid rafts) have lipid compositions, which differ from their surrounding membrane. They have been implicated in cell signaling, cell adhesion, and cholesterol homeostasis. Estimates of their size vary from 40 to 350 nm in diameter depending on the study and cell type used. Rafts are enriched in glycosphingolipids and cholesterol and appear to be in a more ordered lipid phase. Although there is some knowledge of their function in cell signaling, less is known about their assembly and dynamics in cells at various temperatures. We use image correlation spectroscopy and dynamic image correlation spectroscopy to study the clustering and diffusion of glycosylphosphatidylinositol (GPI)-anchored proteins within the plasma membrane of living cells at various temperatures. We find that GPI-anchored proteins occur both as monomers and in clusters at the cell surface. The propensities to cluster as well as the diffusion coefficient of these clusters are strongly temperature dependent. At 37 degrees C the GPI-anchored proteins are highly dynamic with a lower state of clustering than at lower temperatures.
Authors:
Anja Nohe; Eleonora Keating; Marc Fivaz; F Gisou van der Goot; Nils O Petersen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nanomedicine : nanotechnology, biology, and medicine     Volume:  2     ISSN:  1549-9642     ISO Abbreviation:  -     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2007-02-12     Completed Date:  2007-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233142     Medline TA:  Nanomedicine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biological Engineering, Institute of Molecular Biophysics, University of Maine, Orono, Maine, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Cell Membrane / drug effects,  metabolism*
Cercopithecus aethiops
Computer Simulation
Cyclodextrins / administration & dosage*
Glycosylphosphatidylinositols / metabolism*
Kinetics
Models, Biological*
Chemical
Reg. No./Substance:
0/Cyclodextrins; 0/Glycosylphosphatidylinositols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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