Document Detail


Dynamics of E-cadherin and gamma-catenin complexes during dedifferentiation of polarized MDCK cells.
MedLine Citation:
PMID:  10469359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: E-cadherin mediated cell-cell adhesion and hepatocyte growth factor (HGF) are important for renal epithelial morphogenesis. We previously showed that HGF dedifferentiates previously well polarized Madin-Darby canine kidney (MDCK) cell monolayers grown on filters. The regulation of E-cadherin during epithelial dedifferentiation is not known. We hypothesized that E-cadherin mediated cell-cell adhesion is modulated during HGF induced dedifferentiation of MDCK cell monolayers. METHODS: We analyzed E-cadherin/gamma-catenin interaction and distribution during epithelial dedifferentiation in vitro using a model of polarized MDCK cell monolayers treated with HGF. RESULTS: Surface immunoprecipitation experiments showed that HGF increased the amount of cell surface E-cadherin associated with gamma-catenin. Biochemical and morphological examination of the TX-100 solubility of junctional E-cadherin and gamma-catenin in control and HGF treated cells showed an increase in solubility of only E-cadherin during loss of cell polarity. Metabolic labeling of control and HGF treated cells showed that HGF stimulated the synthetic rate of E-cadherin and gamma-catenin molecules. Inulin flux across MDCK cell monolayers increases with HGF treatment. CONCLUSION: These data provide evidence for both the dissociation of E-cadherin molecules from the actin cytoskeleton and an increase in the total number of E-cadherin/gamma-catenin complexes on the cell surface during HGF-induced dedifferentiation of polarized renal epithelium. These data support the hypothesis that E-cadherin function is inhibited by a mechanism of detachment from the actin based cytoskeleton during HGF induced dedifferentiation of polarized renal epithelia.
Authors:
D F Balkovetz; V Sambandam
Related Documents :
11425869 - Role of the f-box protein skp2 in adhesion-dependent cell cycle progression.
10873669 - Identification of a novel beta-catenin-interacting protein.
15053919 - Regulation of e-cadherin expression and beta-catenin/tcf transcriptional activity by th...
9075789 - Expression of e-cadherin, alpha-catenin, beta-catenin and plakoglobin in esophageal car...
11526479 - Igf-ii induces rapid beta-catenin relocation to the nucleus during epithelium to mesenc...
12123599 - Cadherins communicate structural plasticity of presynaptic and postsynaptic terminals.
6861909 - Adhesive responses of fibroblast and neuroblastoma cells to substrata coated with polyv...
7518179 - Bone matrix rgd glycoproteins: immunolocalization and interaction with human primary os...
12766909 - Regulation of cell morphology and adhesion by the tuberous sclerosis complex (tsc1/2) g...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Kidney international     Volume:  56     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  910-21     Citation Subset:  IM    
Affiliation:
Department of Veterans Affairs, Medical Center, and Department of Medicine, Nephrology Research Training Center, University of Alabama at Birmingham, 35294-0007, USA. balkovetz@nrtc.dom.uab.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / physiology
Animals
Cadherins / chemistry,  metabolism*
Cell Adhesion / physiology
Cell Differentiation / physiology*
Cell Membrane / drug effects,  physiology
Cell Polarity / physiology*
Cytoskeletal Proteins / chemistry,  metabolism*
Cytoskeleton / physiology
Desmoplakins
Dogs
Epithelial Cells / cytology,  drug effects,  metabolism
Hepatocyte Growth Factor / pharmacology
Inulin / pharmacokinetics
Kidney / cytology*,  drug effects,  metabolism*
Macromolecular Substances
Models, Biological
Signal Transduction
gamma Catenin
Chemical
Reg. No./Substance:
0/Actins; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Desmoplakins; 0/Macromolecular Substances; 0/gamma Catenin; 67256-21-7/Hepatocyte Growth Factor; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of candesartan cilexetil (TCV-116) in rats with chronic renal failure.
Next Document:  Protection from toxicant-mediated renal injury in the rat with anti-CD54 antibody.