Document Detail

Dynamics of E-cadherin and gamma-catenin complexes during dedifferentiation of polarized MDCK cells.
MedLine Citation:
PMID:  10469359     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: E-cadherin mediated cell-cell adhesion and hepatocyte growth factor (HGF) are important for renal epithelial morphogenesis. We previously showed that HGF dedifferentiates previously well polarized Madin-Darby canine kidney (MDCK) cell monolayers grown on filters. The regulation of E-cadherin during epithelial dedifferentiation is not known. We hypothesized that E-cadherin mediated cell-cell adhesion is modulated during HGF induced dedifferentiation of MDCK cell monolayers. METHODS: We analyzed E-cadherin/gamma-catenin interaction and distribution during epithelial dedifferentiation in vitro using a model of polarized MDCK cell monolayers treated with HGF. RESULTS: Surface immunoprecipitation experiments showed that HGF increased the amount of cell surface E-cadherin associated with gamma-catenin. Biochemical and morphological examination of the TX-100 solubility of junctional E-cadherin and gamma-catenin in control and HGF treated cells showed an increase in solubility of only E-cadherin during loss of cell polarity. Metabolic labeling of control and HGF treated cells showed that HGF stimulated the synthetic rate of E-cadherin and gamma-catenin molecules. Inulin flux across MDCK cell monolayers increases with HGF treatment. CONCLUSION: These data provide evidence for both the dissociation of E-cadherin molecules from the actin cytoskeleton and an increase in the total number of E-cadherin/gamma-catenin complexes on the cell surface during HGF-induced dedifferentiation of polarized renal epithelium. These data support the hypothesis that E-cadherin function is inhibited by a mechanism of detachment from the actin based cytoskeleton during HGF induced dedifferentiation of polarized renal epithelia.
D F Balkovetz; V Sambandam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Kidney international     Volume:  56     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  910-21     Citation Subset:  IM    
Department of Veterans Affairs, Medical Center, and Department of Medicine, Nephrology Research Training Center, University of Alabama at Birmingham, 35294-0007, USA.
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MeSH Terms
Actins / physiology
Cadherins / chemistry,  metabolism*
Cell Adhesion / physiology
Cell Differentiation / physiology*
Cell Membrane / drug effects,  physiology
Cell Polarity / physiology*
Cytoskeletal Proteins / chemistry,  metabolism*
Cytoskeleton / physiology
Epithelial Cells / cytology,  drug effects,  metabolism
Hepatocyte Growth Factor / pharmacology
Inulin / pharmacokinetics
Kidney / cytology*,  drug effects,  metabolism*
Macromolecular Substances
Models, Biological
Signal Transduction
gamma Catenin
Reg. No./Substance:
0/Actins; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Desmoplakins; 0/Macromolecular Substances; 0/gamma Catenin; 67256-21-7/Hepatocyte Growth Factor; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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