Document Detail

Dynamic study of intramembranous particles in human fresh erythrocytes using an "in vitro cryotechnique".
MedLine Citation:
PMID:  16586489     Owner:  NLM     Status:  MEDLINE    
For analyses of dynamic ultrastructures of erythrocyte intramembranous particles (IMPs) in situ, a quick-freezing method was used to stabilize the flow behavior of erythrocytes embedded in vitreous ice. Fresh human blood was jetted at various pressures through artificial tubes, in which the flowing erythrocytes were elongated from biconcave discoid shapes to elliptical ones, and quickly frozen in liquid isopentane-propane cryogen (-193 degrees C). They were freeze-fractured using a scalpel in liquid nitrogen, and routinely prepared for replica membranes. Many IMPs were observed on the protoplasmic freeze-fracture face (P-face) of the erythrocyte membranes. Some control erythrocytes under nonflowing or stationary conditions showed IMPs with their random distribution. However, other jetted erythrocytes under flowing conditions showed variously sized IMPs with much closer distribution. They were also arranged into parallel rows in some parts, and aggregated together. This quick-freezing method enabled for the first time the visualization of time-dependent topology and the molecular alteration of IMPs in dynamically flowing erythrocytes.
Nobuo Terada; Nobuhiko Ohno; Yasuhisa Fujii; Takeshi Baba; Shinichi Ohno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microscopy research and technique     Volume:  69     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-10     Completed Date:  2006-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-5     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 Wiley-Liss, Inc.
Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi,Tamaho, Yamanashi 409-3898, Japan.
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MeSH Terms
Cryoelectron Microscopy / methods
Erythrocyte Deformability*
Erythrocyte Membrane / ultrastructure
Erythrocytes / ultrastructure*
Freeze Fracturing

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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