Document Detail


Dynamic quantitative visualization of single cell alignment and migration and matrix remodeling in 3-D collagen hydrogels under mechanical force.
MedLine Citation:
PMID:  21388676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a live imaging system enabling dynamic visualization of single cell alignment induced by external mechanical force in a 3-D collagen matrix. The alignment dynamics and migration of smooth muscle cells (SMCs) were studied by time lapse differential interference contrast and/or phase contrast microscopy. Fluorescent and reflection confocal microcopy were used to study the SMC morphology and the microscale collagen matrix remodeling induced by SMCs. A custom developed program was used to quantify the cell migration and matrix remodeling. Our system enables cell concentration-independent alignment eliminating cell-to-cell interference and enables dynamic cell tracking, high magnification observation and rapid cell alignment accomplished in a few hours compared to days in traditional models. We observed that cells sense and response to the mechanical signal before cell spreading. Under mechanical stretch the migration directionality index of SMCs is 46.3% more than those cells without external stretch; the dynamic direction of cell protrusion is aligned to that of the mechanical force; SMCs showed directional matrix remodeling and the alignment index calculated from the matrix in front of cell protrusions is about 3 fold of that adjacent to cell bodies. Our results indicate that the mechanism of cell alignment is directional cell protrusion. Mechano-sensing, directionality in cell protrusion dynamics, cell migration and matrix remodeling are highly integrated. Our system provides a platform for studying the role of mechanical force on the cell matrix interactions and thus finds strategies to optimize selected properties of engineered tissues.
Authors:
Yonggang Pang; Xiaoli Wang; Dongkeun Lee; Howard P Greisler
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biomaterials     Volume:  32     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-03-25     Completed Date:  2011-07-26     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3776-83     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / cytology
Cattle
Cell Movement*
Cell Survival
Cells, Cultured
Collagen / chemistry*
Dogs
Equipment Design
Extracellular Matrix / chemistry
Hydrogels / chemistry*
Mechanical Phenomena
Microscopy, Confocal / instrumentation*
Myocytes, Smooth Muscle / cytology*
Single-Cell Analysis / instrumentation
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
R01 HL041272/HL/NHLBI NIH HHS; R01 HL041272-18/HL/NHLBI NIH HHS; R01-HL41272/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hydrogels; 9007-34-5/Collagen
Comments/Corrections

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