Document Detail

Dynamic proteomic and metabonomic analysis reveal dysfunction and subclinical injury in rat liver during restraint stress.
MedLine Citation:
PMID:  19695349     Owner:  NLM     Status:  MEDLINE    
Stress is a risk factor for many diseases. In this study, we used fluorescence difference gel electrophoresis combined MALDI-TOF/TOF and 1H-NMR to monitor the intracellular processes in rat liver at proteomic and metabonomic levels when a rat was treated with restraint stress for 8 weeks. Dynamic changes in 42 proteins and 32 chemical groups were monitored and identified. These proteins and chemical groups were implicated in glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, and the urea cycle. To verify the DIGE result, three proteins including DJ-1, Blvrb and AdoHycase were validated by Western blot. Furthermore, some metabolites related to diseases such as lactate, fatty acid, glucose and homocysteine, were observed to be increasing during 8 weeks of restraint stress. Our data indicated that subclinical hepatic injury occurs during restraint stress, including inhibition of glycolysis and gluconeogenesis in the liver, and dysfunction of fatty acid beta-oxidation. The results suggest a comprehensive map that addresses how functional proteins act on metabolites to produce energy and process materials in rat liver as it responds to restraint stress. Further functional study on these dynamic change proteins and metabolites may lead to better understanding of the mechanisms of stress-induced diseases.
Ming Chen; Yongqing Wang; Yun Zhao; Liqun Wang; Jingbo Gong; Lei Wu; Xiujie Gao; Zhihua Yang; Lingjia Qian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-18
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1794     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-12     Completed Date:  2009-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1751-65     Citation Subset:  IM    
Key Laboratory of Stress Medicine, Tianjing Institute of Hygiene and Environmental Medicine, 1 Dali Road, Heping District, Tianjing 300050, PR China.
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MeSH Terms
Amino Acid Sequence
Blotting, Western
Carbohydrate Metabolism
Electrophoresis, Gel, Two-Dimensional
Homocysteine / blood
Lipid Metabolism
Liver / injuries*,  metabolism*
Magnetic Resonance Spectroscopy
Models, Biological
Molecular Sequence Data
Proteome / genetics,  isolation & purification,  metabolism*
Rats, Wistar
Restraint, Physical / adverse effects*,  physiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stress, Physiological*
Time Factors
Reg. No./Substance:
0/Proteome; 454-28-4/Homocysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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