Document Detail


Dynamic profiles of volatile organic compounds in exhaled breath as determined by a coupled PTR-MS/GC-MS study.
MedLine Citation:
PMID:  20664160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this phenomenological study we focus on dynamic measurements of volatile organic compounds (VOCs) in exhaled breath under exercise conditions. An experimental setup efficiently combining breath-by-breath analyses using proton transfer reaction mass spectrometry (PTR-MS) with data reflecting the behaviour of major hemodynamic and respiratory parameters is presented. Furthermore, a methodology for complementing continuous VOC profiles obtained by PTR-MS with simultaneous SPME/GC-MS measurements is outlined. These investigations aim at evaluating the impact of breathing patterns, cardiac output or blood pressure on the observed breath concentration and allow for the detection and identification of several VOCs revealing characteristic rest-to-work transitions in response to variations in ventilation or perfusion. Examples of such compounds include isoprene, methyl acetate, butane, DMS and 2-pentanone. In particular, both isoprene and methyl acetate exhibit a drastic rise in concentration shortly after the onset of exercise, usually by a factor of about 3-5 within approximately 1 min of pedalling. These specific VOCs might also be interpreted as potentially sensitive indicators for fluctuations of blood or respiratory flow and can therefore be viewed as candidate compounds for future assessments of hemodynamics, pulmonary function and gas exchange patterns via observed VOC behaviour.
Authors:
J King; P Mochalski; A Kupferthaler; K Unterkofler; H Koc; W Filipiak; S Teschl; H Hinterhuber; A Amann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-28
Journal Detail:
Title:  Physiological measurement     Volume:  31     ISSN:  1361-6579     ISO Abbreviation:  Physiol Meas     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-12     Completed Date:  2011-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306921     Medline TA:  Physiol Meas     Country:  England    
Other Details:
Languages:  eng     Pagination:  1169-84     Citation Subset:  IM    
Affiliation:
University Clinic for Anesthesia, Innsbruck Medical University, Anichstr. 35, A-6020 Innsbruck, Austria.
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MeSH Terms
Descriptor/Qualifier:
Acetone / analysis,  chemistry,  isolation & purification
Adult
Breath Tests / methods*
Butadienes / analysis,  chemistry,  isolation & purification
Exhalation*
Female
Gas Chromatography-Mass Spectrometry / methods*
Hemiterpenes / analysis,  chemistry,  isolation & purification
Humans
Kinetics
Male
Noble Gases / metabolism
Organic Chemicals / analysis*,  chemistry*,  isolation & purification
Pentanes / analysis,  chemistry,  isolation & purification
Protons*
Solid Phase Microextraction
Ventilation-Perfusion Ratio
Volatilization
Young Adult
Chemical
Reg. No./Substance:
0/Butadienes; 0/Hemiterpenes; 0/Noble Gases; 0/Organic Chemicals; 0/Pentanes; 0/Protons; 67-64-1/Acetone; 78-79-5/isoprene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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