| Dynamic patterns of apoptosis in the developing chicken heart. | |
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MedLine Citation:
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PMID: 14991705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The outflow tract (OFT) is abnormal in many congenital heart defects. One critical mechanism for morphogenesis of this complex structure is apoptosis. Chicken embryos (stages 19-38; ED4-10) stained with a fluorescent supravital lysosomal dye (LysoTracker Red; LTR) revealed the three-dimensional relationship between structural changes and apoptosis. The LTR staining peaked in the OFT myocardium at stages 27-32, consistent with our previous analyses using other apoptosis assays. While LTR stained under both the pulmonary artery and the aorta, it was most prevalent in the subaortic myocardium before its elimination. Furthermore, LTR staining was most abundant in the myocardium under intensely cytokeratin-positive, thick epicardium. These data support the hypothesis that temporally and spatially restricted apoptosis in the OFT myocardium allows the aorta and pulmonary artery to dock at the appropriate angle and level with the proper ventricle. These data also support a relationship between the differentiating epicardium and cardiomyocyte apoptosis. |
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Authors:
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Katherine S Schaefer; Yong Qiu Doughman; Steven A Fisher; Michiko Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 229 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-01 Completed Date: 2004-10-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 489-99 Citation Subset: IM |
Copyright Information:
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Copyright 2004 Wiley-Liss, Inc. |
Affiliation:
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Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Cell Death Cell Differentiation Chick Embryo Gene Expression Regulation, Developmental* Heart / embryology* In Situ Nick-End Labeling Keratins / metabolism Lysosomes / metabolism Myocardium / cytology, pathology* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL-65314/HL/NHLBI NIH HHS; T32 HD07104/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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