Document Detail

Dynamic patterns of apoptosis in the developing chicken heart.
MedLine Citation:
PMID:  14991705     Owner:  NLM     Status:  MEDLINE    
The outflow tract (OFT) is abnormal in many congenital heart defects. One critical mechanism for morphogenesis of this complex structure is apoptosis. Chicken embryos (stages 19-38; ED4-10) stained with a fluorescent supravital lysosomal dye (LysoTracker Red; LTR) revealed the three-dimensional relationship between structural changes and apoptosis. The LTR staining peaked in the OFT myocardium at stages 27-32, consistent with our previous analyses using other apoptosis assays. While LTR stained under both the pulmonary artery and the aorta, it was most prevalent in the subaortic myocardium before its elimination. Furthermore, LTR staining was most abundant in the myocardium under intensely cytokeratin-positive, thick epicardium. These data support the hypothesis that temporally and spatially restricted apoptosis in the OFT myocardium allows the aorta and pulmonary artery to dock at the appropriate angle and level with the proper ventricle. These data also support a relationship between the differentiating epicardium and cardiomyocyte apoptosis.
Katherine S Schaefer; Yong Qiu Doughman; Steven A Fisher; Michiko Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  229     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-01     Completed Date:  2004-10-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-99     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley-Liss, Inc.
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
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MeSH Terms
Cell Death
Cell Differentiation
Chick Embryo
Gene Expression Regulation, Developmental*
Heart / embryology*
In Situ Nick-End Labeling
Keratins / metabolism
Lysosomes / metabolism
Myocardium / cytology,  pathology*
Time Factors
Grant Support
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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