Document Detail

Dynamic microwave-assisted extraction coupled with salting-out liquid-liquid extraction for determination of steroid hormones in fish tissues.
MedLine Citation:
PMID:  23016883     Owner:  NLM     Status:  Publisher    
In this work, a simple and fast sample pretreatment method was proposed for determination of steroid hormones in fish tissues by coupling dynamic microwave-assisted extraction with salting-out liquid-liquid extraction. The steroid hormones were successively extracted with acetonitrile and water under the action of microwave energy. Subsequently, the extract was separated into an acetonitrile phase and an aqueous phase with ammonium acetate. The acetonitrile phase containing the target analytes was concentrated and determined by LC-MS/MS. The limits of detection for the steroid hormones were in the range of 0.03-0.15 ng g-1. This method was successfully applied to analyze seven kinds of fish tissues, and the recoveries of the steroid hormones for the spiked samples were in the range of 75.3 ± 4.9% - 95.4 ± 6.2%. Compared with the traditional method, the proposed method could reduce the consumption of the organic solvent, shorten the sample preparation time and increase the sample throughput.
Hui Wang; Xiuqing Zhou; Yiqun Zhang; Haiyan Chen; Guijie Li; Yang Xu; Qi Zhao; Weitao Song; Haiyan Jin; Lan Ding
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-28
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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