Document Detail


Dynamic microtubules and endomembrane cycling contribute to polarity establishment and early development of Ectocarpus mitospores.
MedLine Citation:
PMID:  23322087     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array, emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization and provide a foundation for future genetic and genomic investigations of this important developmental process.
Authors:
Jeffrey J Green; Diégo Cordero Cervantes; Nick T Peters; Kyle O Logan; Darryl L Kropf
Related Documents :
24269207 - Distribution of a viscous binder during high shear granulation-sensitivity to the metho...
17434117 - The distribution and life cycle of the dinoflagellate spatulodinium pseudonoctiluca (di...
23414427 - Bluetongue dynamics in french wildlife: exploring the driving forces.
18627817 - The yolk sac in late embryonic development of the stick insect carausius morosus (br.).
14981097 - Mammalian par-1 determines epithelial lumen polarity by organizing the microtubule cyto...
24112707 - Detection of a latent soluble form of membrane-type 1 matrix metalloprotease bound with...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Protoplasma     Volume:  -     ISSN:  1615-6102     ISO Abbreviation:  Protoplasma     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biology, University of Utah, Salt Lake City, UT, 84112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A retrospective evaluation of the effect of hydroxyquinine on RPE thickness.
Next Document:  hPNAS-4 inhibits proliferation through S phase arrest and apoptosis: underlying action mechanism in ...