Document Detail


Dynamic micro- and macrovascular remodeling in coronary circulation of obese Ossabaw pigs with metabolic syndrome.
MedLine Citation:
PMID:  22837170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies from our laboratory showed that coronary arterioles from type 2 diabetic mice undergo inward hypertrophic remodeling and reduced stiffness. The aim of the current study was to determine if coronary resistance microvessels (CRMs) in Ossabaw swine with metabolic syndrome (MetS) undergo remodeling distinct from coronary conduit arteries. Male Ossabaw swine were fed normal (n = 7, Lean) or hypercaloric high-fat (n = 7, MetS) diets for 6 mo, and then CRMs were isolated and mounted on a pressure myograph. CRMs isolated from MetS swine exhibited decreased luminal diameters (126 ± 5 and 105 ± 9 μm in Lean and MetS, respectively, P < 0.05) with thicker walls (18 ± 3 and 31 ± 3 μm in Lean and MetS, respectively, P < 0.05), which doubled the wall-to-lumen ratio (14 ± 2 and 30 ± 2 in Lean and MetS, respectively, P < 0.01). Incremental modulus of elasticity (IME) and beta stiffness index (BSI) were reduced in CRMs isolated from MetS pigs (IME: 3.6 × 10(6) ± 0.7 × 10(6) and 1.1 × 10(6) ± 0.2 × 10(6) dyn/cm(2) in Lean and MetS, respectively, P < 0.001; BSI: 10.3 ± 0.4 and 7.3 ± 1.8 in Lean and MetS, respectively, P < 0.001). BSI in the left anterior descending coronary artery was augmented in pigs with MetS. Structural changes were associated with capillary rarefaction, decreased hyperemic-to-basal coronary flow velocity ratio, and augmented myogenic tone. MetS CRMs showed a reduced collagen-to-elastin ratio, while immunostaining for the receptor for advanced glycation end products was selectively increased in the left anterior descending coronary artery. These data suggest that MetS causes hypertrophic inward remodeling of CRMs and capillary rarefaction, which contribute to decreased coronary flow and myocardial ischemia. Moreover, our data demonstrate novel differential remodeling between coronary micro- and macrovessels in a clinically relevant model of MetS.
Authors:
Aaron J Trask; Paige S Katz; Amy P Kelly; Maarten L Galantowicz; Mary J Cismowski; T Aaron West; Zachary P Neeb; Zachary C Berwick; Adam G Goodwill; Mouhamad Alloosh; Johnathan D Tune; Michael Sturek; Pamela A Lucchesi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-26
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  113     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-05-23     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1128-40     Citation Subset:  IM    
Affiliation:
Center for Cardiovascular and Pulmonary Research, The Heart Center, The Research Institute at Nationwide Children's Hospital, 700 Children’s Drive, Columbus, OH 43205, USA. aaron.trask@nationwidechildrens.org
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity / physiology
Collagen / metabolism
Coronary Circulation / physiology*
Coronary Vessels / metabolism,  physiopathology*
Elastin / metabolism
Male
Metabolic Syndrome X / metabolism,  physiopathology*
Microvessels / metabolism,  physiopathology*
Obesity / metabolism,  physiopathology*
Swine
Grant Support
ID/Acronym/Agency:
HL-056046/HL/NHLBI NIH HHS; HL-062552/HL/NHLBI NIH HHS; HL-092245/HL/NHLBI NIH HHS; HL-63318/HL/NHLBI NIH HHS; RR-013223/RR/NCRR NIH HHS; T32 HL-098039/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-34-5/Collagen; 9007-58-3/Elastin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bimodal collagen fibril diameter distributions direct age-related variations in tendon resilience an...
Next Document:  Respiratory depression in rats induced by alcohol and barbiturate and rescue by ampakine CX717.