| Dynamic in vivo observation of rat islet microcirculation. | |
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MedLine Citation:
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PMID: 8419903 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vivo fluorescent microscopy with direct observation of flow through the islet was used to investigate the islet microcirculation. In urethane-anesthetized rats (n = 18), the pancreas was exposed and an islet was identified under direct microscopy. The vertical illuminator for fluorescent microscopy was turned on and fluorescein-albumin conjugate or fluorescent microspheres were injected intravenously or intraarterially. Each study was videotaped; on slow motion playback, the flow of the conjugate or microspheres was followed through the islet, the islet capillaries, and then to venules exiting the islet. One islet in the head of the pancreas in 12 rats was studied. The arterioles first reached the surrounding mantle of the islet where they divided into capillaries that carried conjugate or microspheres to other portions of the mantle or the core of the islet. Flow of conjugate traversed the core and returned to different portions of the mantle. The fluorescent microsphere study permitted a more detailed study of the pathways followed, the individual microspheres being seen to travel through numerous tortuous pathways through the islet. The flow of microspheres was nonhomogeneous in that individual microspheres in one portion of the islet would stop, then move on, while other microspheres flowed freely. The capillaries joined two to six venules that carried the conjugate or microspheres out of the islet. One or two of the exiting microvessels entered the adjacent acinar microcirculation; the others entered larger collecting venules. In six tail islets studied, the microcirculation was similar to that of the islets in the head of the pancreas.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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Y M Liu; P H Guth; K Kaneko; E H Livingston; F C Brunicardi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pancreas Volume: 8 ISSN: 0885-3177 ISO Abbreviation: Pancreas Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-02-05 Completed Date: 1993-02-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 15-21 Citation Subset: IM |
Affiliation:
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Department of Medicine, VA Medical Center West Los Angeles, CA 90073. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Islets of Langerhans / anatomy & histology, blood supply* Male Microcirculation / anatomy & histology, physiology Microscopy, Fluorescence Microspheres Rats Rats, Sprague-Dawley Regional Blood Flow |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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