| Dynamic green fluorescent protein sensors for high-content analysis of the cell cycle. | |
| | |
MedLine Citation:
|
PMID: 17110183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have developed two dynamic sensors that report cell cycle position in living mammalian cells. The sensors use well-characterized components from proteins that are spatially and temporally regulated through the cell cycle. Coupling of these components to Enhanced Green Fluorescent Protein (EGFP) has been used to engineer fusion proteins that report G1/S and G2/M transitions during the cell cycle without perturbing cell cycle progression. Expression of these sensors in stable cell lines allows high content analysis of the effects of drugs and gene knockdown on the cell cycle using automated image analysis to determine cell cycle position and to abstract correlative data from multiplexed sensors and morphological analysis. |
| | |
Authors:
|
Simon Stubbs; Nick Thomas |
Related Documents
:
|
19305153 - Toxoplasma-induced autophagy: a window into nutritional futile cycles in mammalian cells? 15158053 - Reply: whole-culture synchronization - effective tools for cell cycle studies. 15094373 - Stable sirna-mediated silencing of atm alters the transcriptional profile of hela cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in enzymology Volume: 414 ISSN: 0076-6879 ISO Abbreviation: Meth. Enzymol. Publication Date: 2006 |
Date Detail:
|
Created Date: 2006-11-19 Completed Date: 2006-12-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
|
Languages: eng Pagination: 1-21 Citation Subset: IM |
Affiliation:
|
GE Healthcare, the Maynard Centre, Forest Farm, Whitchurch, Cardiff, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biochemistry / methods* Cell Cycle Cell Line, Tumor G1 Phase G2 Phase Green Fluorescent Proteins / chemistry*, metabolism Humans Image Processing, Computer-Assisted Microscopy, Fluorescence / methods* Models, Biological Protein Structure, Tertiary RNA, Small Interfering / metabolism S Phase Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hydrophobic modifications of Ras proteins by isoprenoid groups and fatty acids--More than just membr...
Next Document: High-content fluorescence-based screening for epigenetic modulators.