Document Detail

Dynamic distribution of NuMA and microtubules in human fetal fibroblasts, developing oocytes and somatic cell nuclear transferred embryos.
MedLine Citation:
PMID:  21406448     Owner:  NLM     Status:  Publisher    
BACKGROUND The nuclear mitotic apparatus (NuMA) plays a central role in the assembly and maintenance of spindle poles. Somatic cell nuclear transfer (SCNT) studies on non-human primates have shown that meiotic spindle removal during enucleation causes depletion of NuMA and the minus-end-directed motor protein (HSET) from the ooplasm, and this in turn leads to failure of embryo development. To determine whether NuMA from somatic cells could compensate for NuMA loss during enucleation, the distribution of NuMA and microtubule organization were investigated in human fibroblasts, developing oocytes and SCNT embryos. METHODS Human fetal fibroblasts, oocytes at various maturation stages and human embryos reconstructed by different SCNT methods were analyzed for NuMA and α-tubulin using immunofluorescent confocal microscopy. RESULTS NuMA was detected in interphase nuclei of fibroblasts and oocytes. During mitosis and meiosis, NuMA relocated to the domain surrounding the two spindle poles. During the enucleation process, NuMA was removed along with the meiotic spindle. At 2 h after injection into a donor cell, transitory bipolar spindles were organized and NuMA was detected in the reformed poles. NuMA could be detected spreading uniformly across the nucleoplasm of one pseudo-pronucleus in SCNT embryos but was excluded from the nucleolus. Regardless of the method used for SCNT (enucleation-injection or injection-pronuclei enucleation), NuMA aggregated and relocated to the reformed spindle poles at metaphase of the first mitotic event. At interphase, NuMA relocated throughout the nucleus in developmentally arrested SCNT embryos. CONCLUSIONS Our results show that donor cell nuclei contain NuMA, which might contribute to the maintenance of spindle morphology in SCNT embryos. Normal spindle and NuMA expression were found in human SCNT embryos at different developmental stages.
Xiaoming Xu; Xin Duan; Changfu Lu; Ge Lin; Guangxiu Lu
Related Documents :
3954068 - Subcellular distribution of the nonspecific esterase in the mouse epididymis with speci...
861938 - Selective incorporation of l-3,4-dihydroxyphenylalanine by s-91 cloudman melanoma in vi...
21558238 - Cell lineage and fate map of the primary somatoblast of the polychaete annelid capitell...
11775058 - Rela, p50 and inhibitor of kappa b alpha are elevated in human metastatic melanoma cell...
23099978 - Effects of the aurora kinases pan-inhibitor sns-314 mesylate on anaplastic thyroid canc...
21115908 - Analysis of the cytotoxic activity of carboplatin and gemcitabine combination.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-15
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  -     ISSN:  1460-2350     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe p...
Next Document:  Growth factor-based therapies provide additional benefit beyond physical therapy in resistant elbow ...