Document Detail


Dynamic culture in a rotating-wall vessel bioreactor differentially inhibits murine T-lymphocyte activation by mitogenic stimuli upon return to static conditions in a time-dependent manner.
MedLine Citation:
PMID:  16384837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Depressed immune function is a well-documented effect of spaceflight. Both in-flight studies and ground-based studies using microgravity analogs, such as rotating wall vessel (RWV) bioreactors, have demonstrated that mitogen-stimulated T lymphocytes exhibit decreased proliferation, IL-2 secretion, and activation marker expression in true microgravity and the dynamic RWV-culture environment. This study investigates the kinetics of RWV-induced T lymphocyte inhibition by monitoring the ability of Balb/c mouse splenocytes to become activated under static culture conditions after concanavalin A (Con A) stimulation in an RWV. Splenocytes were stimulated with Con A and cultured for up to 24 h in the RWV before being allowed to "recover" under static culture conditions in the continued presence of Con A. The T-lymphocyte fraction of splenocytes was assayed during the recovery period for IL-2 secretion, expansion of the T-lymphocyte population, and expression of the activation marker CD25. Our results indicate that CD25 expression was not affected by any duration of RWV exposure. In contrast, proliferation and IL-2 secretion were inhibited by >8 and 12 h of exposure, respectively. Culture in the RWV for 24 h resulted in a near-complete loss of cellular viability during the recovery period, which was not seen in cells maintained in the RWV for 16 h or less. Taken together, these results indicate that for up to 8 h of RWV culture activation is not significantly impaired upon return to static conditions; longer duration RWV culture results in a gradual loss of activation during the recovery period most likely because of decreased T-cell viability and/or IL-2 production.
Authors:
D M Simons; E M Gardner; P I Lelkes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-12-29
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  100     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2007-01-04     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1287-92     Citation Subset:  IM    
Affiliation:
School of Biomedical Engineering, Science, and Health Systems, Drexel University, Commonwealth Hall 7-721, 3142 Chestnut St., Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioreactors*
Cell Proliferation
Cell Survival
Cells, Cultured
Concanavalin A / pharmacology
Interleukin-2 / metabolism
Kinetics
Lymphocyte Activation*
Male
Mice
Mice, Inbred BALB C
Mitogens / pharmacology*
Rotation
Spleen / cytology,  drug effects,  metabolism
T-Lymphocytes / drug effects*,  metabolism
Weightlessness Simulation / instrumentation
Chemical
Reg. No./Substance:
0/Interleukin-2; 0/Mitogens; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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