Document Detail


Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts.
MedLine Citation:
PMID:  19232717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue engineering is an alternative approach for the preparation of small-diameter (<6mm) vascular grafts due to the potential to control thrombosis, anastomotic cellular hyperplasia and matrix production. This control also requires the maintenance of graft patency in vivo, appropriate mechanical properties and the formation of a functional endothelium. As a first step in generating such tissue-engineered vascular grafts (TEVGs), our objective was to develop a tissue-engineered construct that mimicked the structure of blood vessels using tubular electrospun silk fibroin scaffolds (ESFSs) with suitable mechanical properties. Human coronary artery smooth muscle cells (HCASMCs) and human aortic endothelial cells (HAECs) were sequentially seeded onto the luminal surface of the tubular scaffolds and cultivated under physiological pulsatile flow. The results demonstrated that TEVGs under dynamic flow conditions had better outcome than static culture controls in terms of cell proliferation and alignment, ECM production and cell phenotype based on transcript and protein level assessments. The metabolic activity of HCASMCs present in the TEGs indicated the advantage of dynamic flow over static culture in effective nutrient and oxygen distribution to the cells. A matrigel coating as a basement membrane mimic for ECM significantly improved endothelium coverage and retention under physiological shear forces. The results demonstrate the successful integration of vascular cells into silk electrospun tubular scaffolds as a step toward the development of a TEVG similar to native vessels in terms of vascular cell outcomes and mechanical properties.
Authors:
Xiaohui Zhang; Xiuli Wang; Vinny Keshav; Xiaoqin Wang; Jacqueline T Johanas; Gary G Leisk; David L Kaplan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-02-20
Journal Detail:
Title:  Biomaterials     Volume:  30     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-06-24     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3213-23     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / cytology
Biological Markers / metabolism
Bioreactors
Blood Vessel Prosthesis*
Bombyx
Cell Culture Techniques / instrumentation,  methods*
Coculture Techniques
Coronary Vessels / cytology
Endothelial Cells / cytology,  physiology
Female
Glucose / metabolism
Humans
Silk / metabolism*
Tissue Culture Techniques / instrumentation,  methods
Tissue Engineering / methods*
Tissue Scaffolds*
Young Adult
Grant Support
ID/Acronym/Agency:
P41//PHS HHS; P41 EB002520-05/EB/NIBIB NIH HHS; R01 EB003210-04/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Silk; 50-99-7/Glucose
Comments/Corrections

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