Document Detail


Dynamic contrast-enhanced MRI using macromolecular contrast media for monitoring the response to isolated limb perfusion in experimental soft-tissue sarcomas.
MedLine Citation:
PMID:  15480945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to evaluate the potential of dynamic contrast-enhanced MRI for quantitative characterization of tumor microvessels and to assess the microvascular changes in response to isolated limb perfusion with TNF-alpha and melphalan. Dynamic contrast-enhanced MRI was performed in an experimental cancer model, using a macromolecular contrast medium, albumin-(Gd-DTPA)45. Small fragments of BN 175, a soft-tissue sarcoma, were implanted in 11 brown Norway (BN) rats. Animals were assigned randomly to a control (Haemaccel) or drug-treated group (TNF-alpha/melphalan). MRI was performed at baseline and 24 h after ILP. The transendothelial permeability (K(PS)) and the fractional plasma volume (fPV) were estimated from the kinetic analysis of MR data using a two-compartment bi-directional model. K(PS) and fPV decreased significantly in the drug-treated group compared to baseline (p<0.05). In addition, K(PS) post therapy was significantly lower (p<0.05) in the drug-treated group than in the control group. There was no significant difference in fPV between the drug-treated and the control group after therapy. Tumor microvascular changes in response to isolated limb perfusion can be determined after 24 h by dynamic contrast-enhanced MRI. The data obtained in this experimental model suggest possible applications in the clinical setting, using the appropriate MR contrast agents.
Authors:
A Preda; P A Wielopolski; T L M Ten Hagen; M van Vliet; J F Veenland; G Ambagtsheer; S T van Tiel; M W Vogel; A M M Eggermont; G P Krestin; C F van Dijke
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2004-10-10
Journal Detail:
Title:  Magma (New York, N.Y.)     Volume:  17     ISSN:  0968-5243     ISO Abbreviation:  MAGMA     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-02-10     Completed Date:  2005-06-16     Revised Date:  2006-04-24    
Medline Journal Info:
Nlm Unique ID:  9310752     Medline TA:  MAGMA     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  296-302     Citation Subset:  IM    
Affiliation:
Department of Radiology, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015, GD, Rotterdam, The Netherlands. a.huurman@erasmusmc.nl
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MeSH Terms
Descriptor/Qualifier:
Albumins / diagnostic use*
Animals
Antineoplastic Combined Chemotherapy Protocols / administration & dosage
Chemotherapy, Cancer, Regional Perfusion / methods
Contrast Media*
Extremities / blood supply
Gadolinium DTPA / diagnostic use*
Image Enhancement / methods*
Macromolecular Substances / diagnostic use
Magnetic Resonance Imaging / methods*
Male
Melphalan / administration & dosage*
Prognosis
Rats
Rats, Inbred BN
Sarcoma, Experimental / diagnosis*,  drug therapy*
Treatment Outcome
Tumor Necrosis Factor-alpha / administration & dosage*
Chemical
Reg. No./Substance:
0/Albumins; 0/Contrast Media; 0/Macromolecular Substances; 0/Tumor Necrosis Factor-alpha; 0/albumin-(gadolinium-DTPA); 148-82-3/Melphalan; 80529-93-7/Gadolinium DTPA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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