Document Detail

Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats.
MedLine Citation:
PMID:  19799967     Owner:  NLM     Status:  MEDLINE    
Transplantation of bone marrow stromal cells (BMSCs) improves animal neurological functional recovery after stroke. But the mechanism remains unclear. As cell cycle machinery plays an important role in stroke, we investigated the dynamic changes of cell cycle elements in a rat model of middle cerebral artery occlusion. We found the cell cycle markers, cdk4 along with its activator cyclin D1, and proliferating cell nuclear antigen (PCNA), increased after brain ischemia-reperfusion. Phosphorylation of the retinoblastoma protein (pRb, on ser-795), the cyclin D/cdk4 complex mutual target, was upregulated accordingly. However, intravenously administrated BMSCs facilitated cyclin D1, cdk4, and PCNA decrease in the ischemic cortex. Meanwhile, phospho-pRb (ser-795) was completely inhibited. On the contrary, endogenous cdk inhibitor p27 reduced before but enhanced after BMSCs treatment. These findings suggested BMSCs might modulate cell cycle progression in injured brain via downregulation of the cyclin D1/cdk4/pRb pathway as well as upregulation of p27 level. These results provide another way by which BMSCs may contribute to the recovery from stroke.
Kefu Cai; Qing Di; Jingping Shi; Yingdong Zhang
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Publication Detail:
Type:  Journal Article     Date:  2009-09-30
Journal Detail:
Title:  Neuroscience letters     Volume:  467     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-02-25     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  15-9     Citation Subset:  IM    
Department of Neurology, Nanjing Brain Hospital, Nanjing Medical University, No. 264 Guangzhou Road, Nanjing 210029, China.
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MeSH Terms
Bone Marrow Transplantation*
Brain Ischemia / physiopathology*,  surgery*
Cell Cycle / physiology*
Cerebral Cortex / physiopathology,  surgery
Cyclin D1 / metabolism
Cyclin-Dependent Kinase 4 / metabolism
Disease Models, Animal
Infarction, Middle Cerebral Artery / physiopathology,  surgery
Proliferating Cell Nuclear Antigen / metabolism
Rats, Sprague-Dawley
Retinoblastoma Protein / metabolism
Signal Transduction
Stroke / physiopathology,  surgery
Stromal Cells / transplantation*
Reg. No./Substance:
0/Ccnd1 protein, rat; 0/Proliferating Cell Nuclear Antigen; 0/Retinoblastoma Protein; 136601-57-5/Cyclin D1; EC protein, rat; EC Kinase 4

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