Document Detail


Dynamic cell fractionation and transportation using moving dielectrophoresis.
MedLine Citation:
PMID:  17702529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study presents a new cell manipulation method using a moving dielectrophoretic force to transport or fractionate cells along a microfluidic channel. The proposed moving dielectrophoresis (mDEP) is generated by sequentially energizing a single electrode or an array of electrodes to form an electric field that moves cells continuously along the microchannel. Cell fractionation is controlled by the applied electrical frequency, and cell transportation is controlled by the interelectrode activation time. The applicability of this method was demonstrated to simultaneously fractionate and transport Saccharomyces cerevisiae yeast cells, both viable and nonviable, by operating at conditions under which the cells were subjected to positive and negative dielectrophoresis, respectively. Compared to the conventional dielectrophoresis (cDEP and traveling wave dielectrophoresis (twDEP), moving dielectrophoresis allows cells to be separated on the basis of the real part of the Clausius-Mossotti factor, as in cDEP, but yet allows the direct transportation of separated cells without using fluid flow, as in twDEP. This dielectrophoresis technique provides a new way to manipulate cells and can be readily implemented on programmable multielectrode devices.
Authors:
Chin Hock Kua; Yee Cheong Lam; Isabel Rodriguez; Chun Yang; Kamal Youcef-Toumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-17
Journal Detail:
Title:  Analytical chemistry     Volume:  79     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-14     Completed Date:  2007-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6975-87     Citation Subset:  IM    
Affiliation:
Singapore-MIT Alliance, Nanyang Technological University, Singapore 639798.
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MeSH Terms
Descriptor/Qualifier:
Cell Fractionation / methods*
Electrophoresis
Microfluidics* / instrumentation
Saccharomyces cerevisiae / chemistry,  cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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