| Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy. | |
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MedLine Citation:
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PMID: 21456868 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains: wild type (MO6-24∕O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner. |
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Authors:
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Seungrag Lee; Young Ran Kim; Ji Yong Lee; Joon Haeng Rhee; Chang-Soo Park; Dug Young Kim |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of biomedical optics Volume: 16 ISSN: 1560-2281 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: - |
Other Details:
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Languages: ENG Pagination: 036004 Citation Subset: - |
Affiliation:
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Gwangju Institute of Science and Technology, Department of Information and Communications, 1 Oryong-dong, Buk-gu, Gwangju, 500-712 Republic of KoreaDongshin University, Department of Oriental Medicine Materials, 252 Daeho-dong, Naju, Jeonnam, 520-714 Republic of KoreaChonnam National University Medical School, Clinical Vaccine R&D Center, Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Republic of KoreaYonsei University, Department of Physics, 262 Seongsanno, Seodaemun-gu, Seoul, 120-749 Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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