Document Detail


Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy.
MedLine Citation:
PMID:  21456868     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains: wild type (MO6-24∕O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.
Authors:
Seungrag Lee; Young Ran Kim; Ji Yong Lee; Joon Haeng Rhee; Chang-Soo Park; Dug Young Kim
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  16     ISSN:  1560-2281     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  -    
Other Details:
Languages:  ENG     Pagination:  036004     Citation Subset:  -    
Affiliation:
Gwangju Institute of Science and Technology, Department of Information and Communications, 1 Oryong-dong, Buk-gu, Gwangju, 500-712 Republic of KoreaDongshin University, Department of Oriental Medicine Materials, 252 Daeho-dong, Naju, Jeonnam, 520-714 Republic of KoreaChonnam National University Medical School, Clinical Vaccine R&D Center, Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Republic of KoreaYonsei University, Department of Physics, 262 Seongsanno, Seodaemun-gu, Seoul, 120-749 Republic of Korea.
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