Document Detail


Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closure.
MedLine Citation:
PMID:  18184725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dorsal closure is a paradigm epithelial fusion episode that occurs late in Drosophila embryogenesis and leads to sealing of a midline hole by bonding of two opposing epithelial sheets. The leading edge epithelial cells express filopodia and fusion is dependent on interdigitation of these filopodia to prime formation of adhesions. Since the opposing epithelia are molecularly patterned there must exist some mechanism for accurately aligning the two sheets across this fusion seam. To address this, we generated a fly in which RFP-Moesin and GFP-Moesin are expressed in mutually exclusive stripes within each segment using the engrailed and patched promoters. We observe mutually exclusive interactions between the filopodia of engrailed and patched cells. Interactions between filopodia from matching cells leads to formation of tethers between them, and these tethers can pull misaligned epithelial sheets into alignment. Filopodial matching also occurs during repair of laser wounds in the ventral epithelium, and so this behaviour is not restricted to leading edge cells during dorsal closure. Finally, we characterise the behaviour of a patched-expressing cell that we observe within the engrailed region of segments A1-A5, and provide evidence that this cell contributes to cell matching.
Authors:
Thomas H Millard; Paul Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-09
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  135     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-28     Completed Date:  2008-04-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  621-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1DA, UK. tom.millard@manchester.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism
Body Patterning
Drosophila melanogaster / cytology*,  embryology*
Embryo, Nonmammalian / cytology,  embryology,  metabolism
Embryonic Development*
Epithelium / embryology
Green Fluorescent Proteins / metabolism
Homeodomain Proteins / metabolism
Luminescent Proteins / metabolism
Microfilament Proteins / metabolism
Pseudopodia / metabolism*
Receptors, Cell Surface / metabolism
Recombinant Fusion Proteins / metabolism
Transcription Factors / metabolism
Wound Healing
Grant Support
ID/Acronym/Agency:
074867//Wellcome Trust; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Homeodomain Proteins; 0/Luminescent Proteins; 0/Microfilament Proteins; 0/Receptors, Cell Surface; 0/Recombinant Fusion Proteins; 0/Transcription Factors; 0/engrail protein, Drosophila; 0/patched receptors; 0/yellow fluorescent protein, Bacteria; 144131-77-1/moesin; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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