Document Detail


Dynamic regulation of vascular myosin light chain (MYL9) with injury and aging.
MedLine Citation:
PMID:  22003410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Aging-associated changes in the cardiovascular system increase the risk for disease development and lead to profound alterations in vascular reactivity and stiffness. Elucidating the molecular response of arteries to injury and age will help understand the exaggerated remodeling of aging vessels.
METHODOLOGY/PRINCIPAL FINDINGS: We studied the gene expression profile in a model of mechanical vascular injury in the iliac artery of aging (22 months old) and young rats (4 months old). We investigated aging-related variations in gene expression at 30 min, 3 d and 7 d post injury. We found that the Myosin Light Chain gene (MYL9) was the only gene differentially expressed in the aged versus young injured arteries at all time points studied, peaking at day 3 after injury (4.6 fold upregulation (p<0.05) in the smooth muscle cell layers. We confirmed this finding on an aging aortic microarray experiment available through NCBI's GEO database. We found that Myl9 was consistently upregulated with age in healthy rat aortas. To determine the arterial localization of Myl9 with age and injury, we performed immunohistochemistry for Myl9 in rat iliac arteries and found that in healthy and injured (30 days post injury) arteries, Myl9 expression increased with age in the endothelial layers.
CONCLUSIONS/SIGNIFICANCE: The consistent upregulation of the myosin light chain protein (Myl9) with age and injury in arterial tissue draws attention to the increased vascular permeability and to the age-caused predisposition to arterial constriction after balloon angioplasty.
Authors:
Lina A Shehadeh; Keith A Webster; Joshua M Hare; Roberto I Vazquez-Padron
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-07
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-10-17     Completed Date:  2012-02-02     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e25855     Citation Subset:  IM    
Affiliation:
Division of Cardiology, Department of Medicine, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, United States of America. lshehadeh@med.miami.edu
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism*,  physiology
Animals
Aorta / cytology,  injuries,  metabolism,  physiology
Arteries / cytology,  injuries*,  metabolism,  physiology*
Endothelial Cells / metabolism
Iliac Artery / cytology,  injuries,  metabolism,  physiology
Myosin Light Chains / genetics,  metabolism*
Rats
Time Factors
Transcription, Genetic
Transcriptome
Vasoconstriction
Grant Support
ID/Acronym/Agency:
1K01HL096413-01/HL/NHLBI NIH HHS; K01 HL096413/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Myosin Light Chains
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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